Study On Substantial Basis And Pharmacokinetic Of Antibiotic Couple Herb Of Lepidium Sativum-Pheretima Asperfillm

Lepidium sativum which is a traditional medicine of Xinjiang Uygur, is a Lepidium plant of Brassicaceae. It often cure gastrointestinal diseases with Pheretima asperfillm. So far, the chemical constituents and pharmacokinetics research of Lepidium sativum is still unknown, only some pharmacodynamic studies for its seed which has some pharmacology effects like expansion of coronary artery, promote ulcer healing and broad-spectrum antimicrobial. In this thesis, We selected Lepidium sativum-Pheretima asperfillm as the research target aim to find the antibacterial activity substance. The chemical constituents of Lepidium sativum was separated and analyzed to obtain the index components and establish its quality control method; Pharmacokinetics study for Lepidium sativum-Pheretima asperfillm was done in vivo, then observe the dynamic change of chemical constituents and explore its substantial basis and interaction pattern.The main contents and results of this thesis were as follows:1) A series of chemical analysis methods are used to study the chemical constituents of Lepidium sativum, such as alcohol extraction, system solvent extraction, macroporous adsorption resin, silica column chromatography, open ODS column and preparation of HPLC method.The results showed that:through a series of chemical methods,6compounds are obtained from Lepidium sativum,2compounds are obtained from petroleum ether fraction which are beta sitosterol and beta daucosterol,1compound is obtained from ethyl acetate fraction which is HHW,3compounds are obtained from n-butanol fraction which are Protocatechuic acid, Myricetrin and Osthole.2) Establish index components of Lepidium sativum by HPLC.The results showed that:HHW, Myricetrin and Osthole can be used for Lepidium sativum index components and the established HPLC method is suitable for control its quality.3) Antibacterial peptide are obtained from Pheretima asperfillm by precipitate, dialyse, purify methods, which were chosen to take some invitro inhibition tests.The results showed that:the method is suitable for antibacterial peptide extracted and purified, and the antibacterial peptide has some inhibitory actions for Escherichia coli and Staphylococcus aureus.4) The pharmacokinetics process of Myricetrin, Osthol and HHW was investigated through orally adminitrated Lepidium sativum and Lepidium sativum-Pheretima asperfillm in nomal rats by the above method. DAS2.0pharmacokinetic software were used to handle the data.The results were as follows:①The pharmacokinetics process in nomal rats of Myricetrin, Osthol, HHW after oral administration in line with a two-compartment open model.②Pharmacokinetics parameters of myricetrin change as follows:Cmax, Ka, Tmax,V1/F were increased, AUC, a were decreased. Antibacterial peptide could accelerate the absorption, reduce the distribution, achieve rapid clearance rate of Myricetrin.③Pharmacokinetics parameters of Osthol change as follows:a, Ka, CL/F, Cmax were increased, Tmax, V1/F, t1/2α were decreased. Antibacterial peptide could accelerate the absorption, metabolism rate of osthol, to make drugs are absorbed quickly.④Pharmacokinetic parameters of HHW change as follows:Cmax, Tmax, a, Ka were increased, CL/F was decreased. Antibacterial peptide could accelerate the absorption, metabolism rate of HHW.