Study On The Effects And Mechanism Of Chinese Medicine Liyan Oral Liquid On The Animal Models With Acute And Chronic Pharyngitis

Experiment Objective:The acute and chronic pharyngitis for clinical common diseases, its incidence is high, the symptoms stubborn, easy to recur. From clinical drug experience, traditional Chinese medicine is about the unique curative effect. Chinese Medicine Yanling Oral Liquid is a Chinese medicinal preparation, which developed by304Drug clinical Institute of the people’s Liberation Army General Hospital recently. The Chinese Medicine Yanling Oral Liquid has the effect of bring down febrile, detoxifies, promoteing the secretion of saliva or body fluid, profiting pharyngitis relieving cough, reducing sputum. Therefore it is widely used in clinical applies:it is applied to the acute and chronic pharyngitis, cold fever, tonsil inflammation and so on. But at the moment the acute and chronic pharyngitis targeted less animal models, especially chronic pharyngitis reported little animal model, and have not see the bacterial infection caused by chronic pharyngitis animal model, it set up on the animal disease model is very important.In this research we designed the main pharmacodynamics research of Chinese medicine Liyan oral liquid preparation, Explore to establish the model of acute and chronic pharyngitis,observationon on curative effects of Chinese medicine Liyan oral liquid on the animal models with acute and chronic pharyngitis. Explore on Mechanism with cute of Chinese medicine Liyan oral liquid on acute and chronic pharyngitis, its anti-inflammation mechanism in order to offer pharmacological and experiments basis for its safe and effective use in clinic.Experimental research content:part I The rats were used in this study to establish the model of acute and chronic pharyngitis, induced by directly applied into the pharyngeal mucosa with beta-hemolytic streptococci staphylococcus aureus. Make sure bacterium suspension、days、concentration of bacterium、Injection frequency several later the general condition and the index of the laboratory examination were observed including Symptoms and physical signs and hematology Immunohistochemial method etc.part II Main pharmacodynamics research on Chinese medicine Liyan oral liquid1.Antibacteral testing in vitro:The antimicrobial effect of Chinese medicine Liyan oral liquid against bacterium and fungus were tested by tube double dilution method, and to measure the minimum inhibitory concentration(MIC).2.The Anti-inflammatory and detumecent were investigated by dimethylbenzene caused mouse ear swelling, cotton caused rats granuloma swellin, carrageen caused rats paw edemaing,and detumecent effects were investigated by leucocyte strolling induced by CMC-Na in mice3.Chinese medicine Liyan oral liquid’s Cough and expectorant effects were evaluated by Mice tracheal phenol red shedding and Mice from ammonia water cough.4.The pain effects were evaluated by hot plate test in mice and acetic acid leucocyte strolling test in mice.part III Study on the effects and mechanism of Chinese medicine Liyan oral liquid on the animal models with acute and Chronic pharyngitis:1.Study on the effects and mechanism of Chinese medicine Liyan oral liquid on the animal models with acute pharyngitis:The rats were used in this study to establish the model of acute pharyngitis, induced by directly applied into the pharyngeal mucosa with beta-hemolytic streptococci at first. Then, to treat several groups The rats were given Chinese Medicine Yanling Oral Liquid into stomach.2.Study on the effects and mechanism of Chinese medicine Liyan oral liquid on the animal models with Chronic pharyngitis:The rats were used in this study to establish the model of chronic pharyngitis, induced by directly applied into the pharyngeal mucosa with beta-hemolytic streptococci at first. Then, to treat several groups The rats were given Chinese Medicine Yanling Oral Liquid into stomach. Then Observing the morphological changes of pharyngeal mucous membrane of SD rats by optic microscope after HE stained In addition Observing the expression of serum ICAM-1were evaluated by enzyme-linked immuno sorbent assay (ELISA); CRP were evaluated by ABC immunohistochemistry IL-1β, IL-6β and TNF-α were evaluated by radioimmunoassay (RIA), effectsof pathologic morphology.Experiment Results:1. The result showed that the pathologic changes of pharyngeal portion of these rats were similar to acute and chronic pharyngitis. It was suggested that these rats might be serve as animal model for acute and chronic pharyngitis.2. Chinese medicine Liyan oral liquid Have the strong bacteriostasis function to beta-hemolytic streptococci and streptococcus pneumoniae. Have the good bacteriostasis function to staphylococcus aureus, Klebsiella pneumoniae. Have the weak bacteriostasis function to staphylococcus epidermidis Candida albicans and escherichia coli, pseudomonas aeruginosa, staphylococcus albus.In vitro test,the high-dose low-dose,middle-dose of Chinese medicine Liyan oral liquid (7.47g/kg、3.74g/kg、1.88g/kg for rats and11.19g/kg、5.60g/kg、2.80g/kg for mice) were designed.The high-dose of Chinese medicine Liyan oral liquid can significantly increase the respiratory tract of mice phenol red shedding quantity.compared to the blank group have significantly difference.(P<0.05), can decrease the times of mice’s writhing response But medium and low doses groups are no significant difference comparing with blank group (P>0.05).The high-dose and middle-dose of Chinese medicine Liyan oral liquid can be significantly decreased the leucocyte strolling in mice with high middle dosage, compared to the blank group have significantly difference (P<0.05), can extend the dose group of mice cough the incubation period, reduce the number of cough. Compared to the model blank group have significantly difference.(P<0.05或P<0.01), can increase the threshold value of pain. are significant difference comparing with blank group(P<0.01). Chinese medicine Liyan oral liquid lightened the can be significantly decreased ear edema induced by dimethylbenzene in mice, compared to the blank group have significantly difference(P<0.05或P<0.01), can be significantly decreased granuloma edema induced by cotton in rats, compared to the blank group have significantly difference(P<0.05或P<0.01). can be obviously decreased the paw edema by carrageen in rats, compared to the blank group have significantly difference (P<0.05)3. In Chinese medicine Liyan oral liquid (7.47g/kg、3.74g/kg) groups with high-and mid-doses, the expression of serum IL-1β,IL-6β,ICAM-land CRP could be obviously inhibited and the pathological changes of model group with acute and Chronic pharyngistis improved in different degrees; In Chinese medicine Liyan oral liquid high-dose group, the expression level of TNF-a could be inhibited.Experiment conclusion:1. This subject is established and the successful copy irritable, chronic pharyngitis SD models in rats, work out the acute and chronic pharyngitis animal models made new modeling methods, and prove good reproducibility.2. Chinese Medicine Liyan Oral Liquid is a Chinese medicinal preparation, which developed by304Drug clinical Institute of the people’s Liberation Army General Hospital recently. The Chinese medicine Liyan oral liquid has the effect of bring down febrile, detoxifies, promoteing the secretion of saliva or body fluid, profiting pharyngitis relieving cough, reducing sputum. The previous pharmacological research indicated that the Chinese medicine Liyan oral liquid have strong action in anti-bacterial, antiphlogistic, profited pharyngitis, expectorants, antitussives,analgesic. Therefore it is widely used in clinical applies:it is applied to the acute and chronic pharyngitis, cold fever, tonsil inflammation and so on. Indicate that Chinese medicine Liyan oral liquid vial has a good curative effect in treating acute and chronic pharynxgitis. The therapeutic mechanisms may be that to adjust the expression of cytokines and to inhibition the changes of histological sections with lesion.