Proliferation Inhibiting And Apoptosis Inducing Effects Of Extracts Of Albizzia Julihrissin Durazz. On Rat Glioma Cell C6

Glioma, the most common primary malignant brain tumor within the neuronal ectoderm, accounts for about50%of intracranial tumors. The mean survival of a patient with glioma is only9-12months. According to the ranking of disease mortality from World Health Organization(WHO) in1998, cause of death from wicked gliomas ranked the second below34years old patients with tumor, and the third between35to54years tumor patients. Due to the aggressive and invasive growth of this tumor and its capacity to infiltrate diffusely into regions of normal brain is the main characteristic of glioma, which results in high rate of incidence, recurrence and mortality, but low cure rate. Thus, it’s very important to find methods treating gliomas. Due to the frequent location of supratentorial gliomas near or within the so-called "eloquent’" area, rendering difficult total surgical extirpation. Radiotherapy often damages tumor and surrounding normal tissue by radioactive rays. It’s very important to find the safe and inocuity medicine treating gliomas. Traditional Chinese Medicine (TCM), has particular advantage, was used for prevention and treatment of cancer.This work respectively extracted9TCMs including Portnhica grandiflora Hooks.(Ban zhi Han), Poly aviculare L.(Bian xu), Flower of Albizzia julibrissin Durazz(AJDF), Bark of Albizzia julibrissin Durazz(AJDB), Bupleurum chinensis DC(Chai hu), Arisaema heterophyllum Blume(Tian nan xing), Hedyotis diffusa(She she cao), Jurinea(Xuan fu hua), Gardenia jasminoides Ellis(Zhi zi) with distilled water. The extracted soup was vacuum extraction filtered and concentrated, the last, the concentrated liquid was freeze-dryed. The effects of extracts on cell viability of rat glioma cell lines C6were measured by MTT assays. The results showed that only the flower and bark of Albizzia julibrissin Durazz could inhibit markedly the proliferation of C6cell lines. So, we further researched that apoptosis-inducing and cell cycle distribution effects of these two medicines on C6cell lines. The conclusions as follows:l.The water extracts of AJDF and AJDB could inhibit the proliferation of C6cell lines, and the the growth inhibiting effects had significant concentration-/time-dependent relationships. The ICso values of AJDF were respectively305μg/mL,145μg/mL,15μg/mL at24h,48h,72h. The IC50values of AJDB were respectively265 μg/mL,195μg/mL,95μg/mL at24h,48h,72h.2.The water extracts of AJDF and AJDB could induce C6cell lines apoptosis. Compared with the control group,200μg/mL、250μg/mL、300μg/mL AJDF maked cell apoptosis rates increased1.4%、3.4%、13.3%,200μg/mL、250μg/mL AJDB maked cell apoptosis rates increased2.1%、13.7%after24h.3.The water extracts of AJDF and AJDB could make C6cell lines S phase accumulation. At24h,300μg/mL、400μg/mL AJDF maked the proportion of S phase cell increase from32.169%to46.990%、49.623%.300μg/mL、400μg/mLAJDB maked the percent of S phase cell increase from30.107%to39.910%、45.395%.4.Within extraction of AJDB, only remaining aqueous could not inhibit the proliferation of C6cell lines, the others could inhibit the proliferation of C6cell lines, the order from strong to weak were n-butanol phase, crude extract, petroleum ether phase, chloroform phase, ethyl acetate phase.