Coronary Heart Disease With Blood Stasis Syndrome And VWF Gene Polymorphism Correlation Study

Objective:Discussion on coronary heart disease with blood stasis syndrome type and intron10of vWF gene C/T polymorphism.Methods:coronary heart disease patients with blood stasis syndrome in coronary heart disease100cases, non blood stasis syndrome and healthy controls each of them50cases, both peripheral anticoagulated whole blood extracted from leukocytes DNA, polymerase chain reaction PCR fragments, gel electrophoresis for detection of vWF gene M+/M-polymorphism.Result:1. sample VWF gene Mspl genotype distribution of M+M+genotype6%, M+M-genotype32.5%, M-M-genotype61.5%, C, T allele frequencies were22.25%and77.75%, the distribution of the observed values and predicted value, the difference was not statistically significant (P=0.853).2. vWF genotype and allele, in the group of coronary heart disease, coronary heart disease with blood stasis syndrome in coronary heart disease group and non blood stasis syndrome group distribution compared with healthy controls, the differences were statistically significant (P=0.013,0.003,0.045); and in coronary heart disease with blood stasis syndrome in coronary heart disease group and non blood stasis syndrome group, blood stasis syndrome in coronary heart disease group and coronary heart disease were compared between groups, distribution of no significant difference (P=0.538,0.782). The M-M genotype may be independent risk factor for coronary heart disease, M-M gene with coronary heart disease with blood stasis syndrome type OR2.833(1.405-5.710)(P=0.003); M-M genotypes in blood stasis syndrome of coronary heart disease than the OR 2.263(P=0.045). Alleles C, T in the four groups distribution difference does not have statistical significance (P>0.05)3. coronary heart disease with blood stasis syndrome in three genotypes of blood stasis syndrome integral form than the differences were not statistically significant,(X2=0.737, P=0.947>0.05), so don’t think vWF Mspl gene polymorphism and blood stasis syndrome of apparent correlation integral.Conclusion:1. study general subjects genotype, allele frequency distribution of the overall population of consistent with local.2. vWF Mspl gene polymorphism, M-M genotype may be independent risk factor for coronary heart disease, but the three genotypes are not an independent risk factor for coronary heart disease with blood stasis syndrome. And in patients with blood stasis syndrome without obvious correlation integral.3. alleles C, T had no correlation with blood stasis syndrome in coronary heart disease.