| ObjectiveThe black plaster "active analgesic paste" formulation of reform, preparation of good curative effect, the skin permeability is good, the side effect is small external traditional Chinese medicine compound preparation" active analgesic plaster". On active analgesic plaster in purple Sal-Ammoniae for processing technology of comparative study on prescription drugs, extraction technology, technology of preparation and formation of, and part of quality analysis, experimental research on the factors influencing the development of active analgesic plaster, as a preliminary study.Method:1. Purple Sal-Ammoniae processing:a study with sulfur content as the index, uses the ultraviolet spectrophotometric method determination of ammonium chloride in276nm purple raw materials and3kinds of different processed products (water vinegar float cream methodlmethod, water vinegar float cream2method, direct fire preparation method of sulfur in vinegar) comparison of content, select the appropriate purple Sal-Ammoniae processing toxicity removal method.2. Volatile oil extraction process research:Distillation method was used to extract volatile oil, the volatile oil extraction quantity indexes, to soak time (A), water (B), extraction time (D) as a major factor, with L9(34) orthogonal test optimization. In order to accurately understand the volatile oil extraction effect, the volatile oil extractor with xylene extraction method and volatile oil extractor to extract the volatile oil of two kinds of methods are compared.3. Ethanol extraction process research:the monarch drug decocting, kudzu root effective component of Dipsacus saponin VI and puerarin extraction volume, extract content as the index, using high performance liquid chromatography method for the determination of Dipsacus asper saponin VI and the content of puerarin, selection of alcohol concentration, dosage (ml/g), extraction time (H), extraction times as influencing factors, with L9(34) orthogonal test to optimize the ethanol refluxing extraction technology of prescription medicines.4. Study on forming process of4formulations:in the experimental basis, to plaster melted, uniformity, hardness, viscosity score, choice of glycerin, twelve alkyl sodium sulfate, stearicacid, triethanolamine, monoglyceride, lanolin, paraffin as a matrix, using L18(37) orthogonal test optimization active analgesic plaster affixed to the substrate ratio.5. Qufenghuoluo plaster and the original black plaster TLC comparison study:Qufenghuoluo plaster and the original black plasterformulationofpuerarin, teasel, Gleditsiasinensis, rhubarb,C aulis,cinnamon,camphor, sevenmedicinalmaterialswasidentified by TLC.6. Preparation stability influence factor test:The active analgesic plaster placed on the condition of40DEG C, and placed25 degrees C, RH92.5%conditions, to study its plaster moisture absorption, the influence of content determination.Result:1. Sulfur in1-30g/mL concentration range had a good linear relationship (r=0.99995), the average recovery was101.09%, RSD=2.37%(n=6). Purple Sal-Ammoniae health products of sulfur content in for111.05g/g,3kinds of different processed products of sulfur content are:boiled for14.37g/g, water vinegar float cream1for21.11g/g, water vinegar float cream2method is36.90g/g, direct fire vinegar preparation method for42.40g/G. Not only can achieve the processing in addition to poison effect, at the same time ensure efficacy of the drugs, in order to direct fire vinegar processing method of purple Sal-Ammoniae best.2. Plus xylene volatile oil obtained significantly more often than not xylene the volatile oil, description of volatile oil in distillation process may lead to a certain loss. Optimization of the extraction process of volatile oil distillation method for adding medicine:6times the volume of water, soaking1H,4h, extraction.3. Preferred ethanol extraction technology of prescription medicines:adding6times amount of70%ethanol extraction for3times, each time2hours. Teasel saponin VI and puerarin metastasis rate%and%, indicates optimal extraction process of ideal.4. Preferred technology of preparation and formation:lgdryextract, glycerin.0.45g0.06g0.15g, emulsifier, triethanolami ne, stearicacidmonoglyceride0.90g,0.90g0.30g0.90g, lanolin, paraffin, ethyl p-hydroxybenzoate0.01g.5.1. Comparison on the extraction solven、 matrix accessoriesa and preparation technology:The extraction efficiency of menstruum of Qufeng Huoluo patch is high; the performance of its auxiliary material is good; and its preparation technology is reasonable and effective. However, for the traditional black plaster, its extraction efficiency of menstruum is low; the auxiliary material is minium and it is poisonous; the release rate is low and the preparation technology is not stable.2.Comparison of thin-layer chromatography identification:The thin-layer chromatography identification methods for radix dipsaci, radix puerariae, rhubarb, spina gleditsiae, cinnamon, camphor and caulis sinomenii are also simple and have no interference for the negative samples. And the obtained chromatogram spots are clear. However, only the layer spots of radix puerariae can be identified in the traditional black plaster; and for the other medicine materials, no layer spots are illustrated.6. The factors affecting results:Active analgesic plaster samples, in40degree test under conditions of continuous inestigation within10days, the testing results and the test were compared before, there are no obvious change. From the results it can be seen in plaster Nakagawa teasel root saponin VI and the content of Puerarin in the condition of high temperature stability.Active analgesic plaster sample under high humidity condition esay to absorb water, causing the sample to the increased water content, affect the quality of products,sealed packaging, quality assurance and stability, the results can be seen in the plaster Nakagawa teasel root saponin VI and the content of Puerarin in the condition of high temperature stability. Conclusion:Development of active analgesic plaster has a good appearance, the plaster can be applied to the skin to achieve good results. Extraction, preparation process, quality and so on, for its development to lay a good foundation. |
PDF Full Text Request