Detection And Comparison Of Plasma Calprotectin In Different Stages Of Diabetic Retinopathy In Patients With Type2Diabetes Mellitus

Background The neutrophils infiltration and vascular endothelium damage are foundin the patients with diabetic retinopathy （DR）. Calprotectin exist in the cytosoloutside lysoome. It is thought to be a marker of inflammation. The effect ofcalprotectin in the development of DR is still in the study.Objective1. This study was to investigate the contents of plasma calprotectin of DRin patients with type2diabetes mellitus，and approach the effects of calprotectin onthe occurrence of DR.2. To measure the calprotectin levels in plasma withnon-proliferativediabetic retinopathy（NPDR）and proliferative diabetic retinopathy（PDR）respectively，and approach the effects of calprotectin on the developmentofDR.Methods This was a case-control study. Sixty consecutive patients with type2diabetes mellitus from the department of ophthalmology of Wuxi No.2hospital ofNanjing medical university were enrolled in this study. The patients were assigned tonon-DR （NDR） group， non-proliferative DR （NPDR） group and proliferativeDR （PDR） group according to fundus appearance and fundus fluoresceinangiography（FFA）manifestation and twenty patients for each group. Twenty healthysubjects matched in gender，age and blood biochemical indicators were collected asthe normal control group. The periphery blood samples were collected from thesubjects for the detection of plasma calprotectin by ELISA. The plasma calprotectinlevels were compared among different stages of DR and normal subjects. All datawere entered into the computer and analysed by SPSS18.0statistical software. Thedata of calculations were presented as mean±standard（x±s）deviation. Gender differences of the four groups of subjects were compared using the!2test. Thedifferences of the ages，duration of disease，fasting blood glucose concentrations andglycosylated hemoglobin content of the four groups were compared by single factoranalysis of variance. Between any two of the indicators group were compared bySNK-q test. The content of the calprotectin concentration and neutrophil werecompared by Pearson correlation analysis. P <0.05was considered statisticallysignificant.Results The contents of plasma calprotectinwere（57.70±12.29），（72.07±10.14），（87.70±10.37），（94.36±9.40）ng/L in the normal control group，NDR group，NPDR group，PDR group respectively，with a statistically significant differenceamong4groups（F=73.09， P<0.001）. The content of calprotectinin PDR groupshowed a highest value in comparison with normal control group，NDR group andPDR group （q=20.157，10.648，4.497，P<0.01）. The content of calprotectininNPDR groupwas significantly higher than that in NDR group （q=6.216，P<0.01）.No significant correlation between calprotectin concentration and peripheral bloodneutrophil numbers（r=0.052，P=0.659）.Conclusions1. Plasma calprotectin may play a role during the development of DR intype2diabetes mellituspatient.2. The high expression of the plasma calprotectin maybe an important role in the progression of diabetic retinopathy and the severity of thedisease.3. Determinationthe concentration of the plasma calprotectin may serve apotential clinical value of inflammatory markers.