Difference Expssion And Correlations Of Pdgfr-β And COX-2in Colorectal Cancer

Background: Colorectal cancer is one of the most common humangastrointestinal malignant tumor, its incidence and mortality whether indeveloping countries or western developed countries have show clearascendant trend [1]. And the occurrence and development of tumorinvasion and metastasis, is more than a cause, more complexpathophysiological stage of evolution process. Related research foundthat growth factor receptor and related enzymes, etc can participate incancer cell proliferation and differentiation, and form a complex functionsignal network, each other mutual coordination and influence each othercommon involved in the cell signaling, promote the formation anddevelopment of the tumor.Growth factor receptor family platelet derivation growth factorreceptor (platelet-derived growth factor receptor, PDGFR) is receptortyrosine protein family members, after a series of phosphorylation toactivate cell signal transduction pathways, regulating calcium phosphatemetabolism, induction actin reconstruction, antiapoptotic and so on manykinds of effects. In addition, PDGFR-β signal pathway can also promotethe original cell proliferation, mediated basement membrane pipe of theformation, thus induce tumor formation of new blood vessels, directly orindirectly involved in cancer occurrence, development and hit and transfer. An enzyme called cyclooxygenase (cyclooxygenase, COX)-2isthe body of the prostaglandin synthetic enzyme speed limit, can catalyticarachidonic acid metabolism for important second messenger PGs, inrecent years, the study found in the body COX-2a wide variety of tumorare over-active, involved in cancer cells, the evolution of malignanttransformation process. In today’s high-speed development of the medicalfield, especially the development of life science, people successfulimplementation, the completion of the human genome project, and clonedand all human disease related gene, people to the tumor molecularbiological level and its gene level have a more profound understanding of,to the tumor molecular inhibition theory and molecular targets have vastliterature reports, however, further improve the correct diagnosis andearly postoperative clinical efficacy and long-term survival and still needfurther researchObjective: this experiment immunohistochemical method using thejoint test organization and its cut colorectal cancer in PDGFR-β sourceorganization, COX-2expression, preliminary analysis PDGFR-β, COX-2in the function and the large mutual relationship, and further discussed inboth colorectal cancer occurrence, development, and the possiblemechanism by transfer, thus for further research on tumor formationrelated molecular level control mechanism, tumor early proper diagnosisand treatment of cancer drug research and applied to provide the theory basis. Methods: Randomly select specimens of colorectal cancer65patients with a complete clinical and pathological data by radicalsurgery (or palliative) resection, and did not receive any chemotherapy,radiotherapy and biological therapy before surgery;Use SP ofimmunohistochemical group to test65colorectal cancer tissues,anddetect PDGFR, COX-2expression in peripheral tissues. Results:1. Thepositive expression rate of PDGFR-βin colorectal cancer peripheraltissues and colorectal adenomas is69.23%，16.92%. PDGFR-βhighexpression and incidence of colorectal cancer, Dukes stage、TNM stageand malignant behavior are closely related (P<0.05).2.The positiveexpression rate of COX-2in colorectal cancer, peripheral tissues andcolorectal adenomas is78.46%、1.54%. COX-2high expression andincidence of colorectal cancer, invasion, metastasis, histological gradeand other malignant behavior are closely related (P<0.05).Conclusion:1.PDGFR-βand COX-2together participate in the evolutionof colorectal cancer, the high expression of both is tightly related to thedegree of tumor differentiation, depth of invasion, lymph nodemetastasis, Dukes stage, but not associated with the age, gender, tumoroccurrence location and size.2.PDGFR-βand COX-2have a positivecorrelation of high expression in colorectal cancer, suggesting that thetwo may be synergic and superposed in the process of tumorformation.3. The joint detection of PDGFR-βand COX-2expression in colorectal cancer can be treated as an indicator of colorectal cancerprognosis and biological behavior; Both PDGFR-βand COX-2have theexpression in the early stages of colorectal cancer, and this is expected tobecome diagnostic markers.