Expression And Clinical Significance Of MACC1in Human Esophageal Squamous Cell Carcinoma

Objective:1. To investigate whether metastasis-associated in colon cancer-1(MACC1) is expressed in human esophageal squamous cell carcinoma (ESCC), and to compare differences between ESCC and para-cancer;2. To explore relationship and its clinical significance between MACC1expression and clinicopathological characteristics. Materials and methods:1.51pairs of human ESCC tissues and para-cancer tissues were collected from patients who underwent surgical resection between March2011and December2011in the Department of Thoracic Surgery, the Affiliated Hospital of Luzhou Medical College. All patients had no history of chemotherapy, radiotherapy or other anti-cancer therapy and all specimens were confirmed histologically;2. The expression of MACC1mRNA in ESCC and para-cancer was detected by reverse transcription polymerase chain reaction (RT-PCR);3. To confirm the results of RT-PCR, MACC1mRNA expression levels in ESCC and para-cancer were examined by using real-time fluorescence quantitative polymerase chain reaction (qRT-PCR);4. Western-blot (WB) technique was used to investigate the expression of MACC1protein in ESCC and para-cancer; Results:1. RT-PCR results showed that MACC1mRNA was expressed in40cases of ESCC and41cases of para-cancer, the relative amount were0.1865+0.1280and0.2665±0.1791, respectively. Compared to ESCC, values of MACC1mRNA were more highly in para-cancer and the difference was considered statistically significant (P=0.01l). MACC1mRNA expression was significantly up-regulated in ESCC with lymph node metastasis compared to those without lymph node metastasis (0.2135±0.1167vs0.1385±0.1206), and the difference was considered statistically significant (P=0.032). Whereas in the group of gender, age, tumor size, location, differentiation and depth of invasion the difference was considered no statistically significant(P>0.05).2. Similar to the outcome of RT-PCR, qRT-PCR analysis indicated that the expression values of MACC1mRNA in para-cancer were2.3811-fold greater than those in ESCC, and the difference was considered statistically significant (P=0.026). MACC1mRNA expression values in ESCC with lymph node metastasis was1.98times higher than those without lymph node metastasis and the difference was considered statistically significant (P=0.012). Whereas in the group of gender, age, tumor size, location, differentiation and depth of invasion the difference was considered no statistically significant (P>0.05).3. The results of WB suggested that MACC1protein was expressed in42cases of ESCC and46cases of para-cancer, the relative amount were0.4976±0.5647and0.7236±0.5510, respectively. Compared to ESCC, values of MACC1protein were more highly in para-cancer and the difference was considered statistically significant (P=0.043). MACC1protein expression was dramatically increased in ESCC with lymph node metastasis compared to those without lymph node metastasis (0.6244±0.6167vs0.3164±0.4327), and the difference was considered statistically significant (P=0.041). Whereas in the group of gender, age, tumor size, location, differentiation and depth of invasion the difference was considered no statistically significant (P>0.05). Conclusions:1. The ratio of MACC1gene expression in human ESCC and para-cancer was high;2. MACC1gene is related to the easy metastasis characteristics of ESCC, and its abnormal expression may promote metastasis of ESCC.3. MACC1might be serve as an important indicator to determine the metastatic potential and prognosis of ESCC.