Experimental Study On The Hypoglycemic Effects Of Reed Rhizome Ethanol Extract To Diabetic Mice

ObjectiveDiabetes mellitus (DM) is a metabolic disorder syndrome, which resulting from genetic factors, immune disorders, microbial infection and its toxins, radical toxins, spirit and other sorts of pathogenic factors and resulting to islet function decline, insulin resistance and such as sugar, protein, fat, water and electrolyte and so on. Once the control of blood sugar is bad can cause kidney, eye, foot areas such as the failure of pathological changes, and can not be cured. Reed root contains abundant polysaccharide compounds, and includes alcohol, betaine, together YiYi element, free amino acid, lucid asparagus amide,, with high medicinal value. This experiment is through the creation of diabetic mice model, to make a study of diabetic mice lowering blood sugar and other repair function.Methods①The phragmites ethanol extract was phragmites after ethanol extraction, cooling, ethanol precipitation, and distilled water elution steps.②Preparation of diabetic mice model:intraperitoneal injection of freshly prepared alloxan solution (200mg/kg), can not help but water after60hours fasting, tail vein blood after12hours, glucose meter to measure blood sugar values greater than11.1mmol/L for the diabetic mice.③Grouping experimental animals were as follows:normal control group, model group,make a low dose group, make dosage group, reed root in the high-dosage groups, drug control group. The cycle was15days, the daily fill a stomach, stop the animal medicine weight, measuring the fasting blood sugar, the eye socket vein plexus take blood after collecting specimens, cutting the pancreas, liver, take mice kidneys.④The enzyme kinetic tested Glycosylated serum protein (GSP), Total cholestcrol (CHO), Low-density lipoprotein,(LDL-C), Triglyceride (TG), High-density ipoprotein (HDL-C), Glutathione peroxidase (GSH-PX) and Supcroxidc dismutase (SOD), maleicdialdehyde (MDA), total antioxidant capacity (T-AOC), Glutathione (GSH), catalase (CAT). ELISA tested serum insulin (INS), insulin resistance receptor β (IR-B).⑤Immunohistochemical staining method observed the pancreas, liver and kidney tissue repair. Results1.The phragmites ethanol extract dose group, the phragmites high-dose group and the drug control group before and after experimental blood glucose was significantly lower (P<0.01). The phragmites ethanol extract high dose group and the drug in the phragmites control group GSP levels were significantly lower than with the model group (P<0.01); The phragmites ethanol extract high, the phragmites low-dose group and the drug control group GSP content were not significantly different.2.Make a high, middle and low dose groups and drug control group CHO content were significantly lower than the model group (P<0.01);Make a high, middle and low dose group and control group CHO content drugs were no significant difference.Make a high dose group and drug control group TG content were significantly lower than and model group (P<0.01); Make a high dose group and control group in drug TG no significant differences.3.Make a high dose group and drug control group HDLC content were significantly higher in model group (P<0.01);Make a high dose group and drug control group had no significant difference HDLC content.Make a high, middle and low dose groups and drug control group LDLC content were significantly lower than the model group (P<0.01);Make a high, middle and low dose group and control group LDLC content drugs were no significant difference.4.Make a high, middle and low dose groups and drug control group GSH-PX content were significantly higher in model group (P<0.01);Make a high, middle and low dose groups and drug control group GSH-PX content were no significant difference.Make a high dose group and drug control group were significantly higher than SOD content in model group (P<0.01); Make a high dose group and drug control group SOD content no significant difference. Make a high dose group and drug control group were significantly lower than the MDA content model group (P<0.01); Make a high, middle and low dose groups and drug control group had no significant differences between the MDA content.5. Make a high, medium and low doses groups and drug control group T-AOC, GSH, CAT content were significantly higher in model group (P<0.01); Make a high, middle and low dose groups and drug control group T-AOC, GSH, CAT content were no significant difference; Make a high dose group was obviously higher than CAT drug control group (P<0.01). Make a high, middle and low dose groups and drug control group T-AOC, GSH conten there were no significant difference.6. Make a dose group of high, medium and low drugs significantly higher than the control group INS、IR-B content in model group (P<0.01); Make the high, medium and low dose group of drugs and make no significant differences in the control group INS. Make a high dose group of IR-B content is higher than drugs in control group (P<0.01). For the damaged islet cell has B repair function, can improve the islet play to its normal functions, reduce blood sugar.7. Make ethanol extract can promote the liver tissue of the use of glucose, and to reduce blood sugar; At the same time for the damaged kidney has obvious improvement action.Conclusion1.Make a significantly lower ethanol extracts four oxygen pyrimidine diabetic mice induced by blood glucose and GSP content.2.Ethanol extracts oxygen to make four degradable diabetic mice induced with a better control lipid metabolism.3. Make ethanol extracts can make four oxygen pyrimidine diabetic mice induced by effectively using antioxidant effect.4.Make a significant increase in ethanol extracts four oxygen pyrimidine diabetic mice induced by INS, IR-B content; For the damaged islet cell has B repair function, can improve the islet play to its normal functions, reduce blood sugar.5.Make ethanol extracts can make four oxygen pyrimidine diabetic mice induced liver to enhance the use of glucose, and to reduce blood sugar; At the same time for the repair of damaged kidney has obvious effect.