Efect Of Qinghuobaiduyin On The Apotosis Of Intestinal Epithelial Cells In Severely Burned Rats

[Objectives] QHBDY is the clinical prescription of Third Xiangya Hospital,mainly composed of astragalus,honeysuckle,scutellaria, radix and rhubarb. Its main role is in clearing heat and detoxicating promoting blood circulation and removing blood stasis,supplementing qi and nourishing yin,and so on.and Apprarent effects were abtained since applied to clinics in1996.According to the previous research of our laboratory, QHBDY showed a significant regulatory effect on humoral immunity, cellular immune function of severe burn patients. In addition, the regulation is bidirectional.To study the protective effect of QHBDY on small intestinal mucosa of rats and the influence on the apoptosis in small intestinal epithelium.[Methods] The scalded rats were taken as the model45SD rats were employed in the study and were randomly divided into3groups: normal control group(n=5), simple burn group(n=20), QHBDY+burn group(n=20). For the burn group, Small intestinal mucosa samples were repectively token after burn at6h,12h,24h,48h (5rats every time). For the treatment group,20rats were given QHBDY (1.0g/L,1.0ml/100g) by oral infusion before burn, and given the same dose within5minutes after burn..The Bcl-2and Caspase-3expression in small intestinal mucosa was determined byusing immunohistochemistry,and the apoptosis of Small intestinal mucosal tissue in all groups was analysed by TUNEL,,meanwhile,the pathomorphological changes of Small intestinal mucosal tissue was also examined.[Result] The expression levels of Bcl-2at different time points after burn were significantly higher than that in control group(P<0.05).The level of Bcl-2was high to peak between6-12h after injury,low expressed at24h and rebounced to the second peak at48h. The apoptosis rates of Caspase-3at different time points after burn were significantly lower than that in control group.(P<0.05),except the6h group.The level of Caspase-3was lowest between6-12h after burn, but it began to rise at24h after burn.48h later the level of Caspase-3began to decrease. The level of Bcl-2in QHBDY+burn group was higher than that in burn group at most time after burn(P<0.05). Apoptotic index in small intestinal epithelium at different timepoints after burn was significantly higher than that in control group(P<0.05). Apoptotic index was highest at12h after burn and the second highest Apoptotic index was at48h after burn. Apoptotic index in group A was significantly lower than that in group B at most time points after burn(P<0.05). The HE staining revealed that small intestinal epithelium tissues of burn group were damaged obviously after burn but the small intestinal tissues of control group were integrated and had no obvious pathological changes. The damages of small intestinal epithelium tissues of QHBDY+burn group were relieved obviously.[Conclusions] The small intestinal epithelium tissues of SD rats were damaged obviously during the early burning stage, and maybe the apoptosis in small intestinal epithelium cells is one of the most importantcauses in the damage of small intestinal epithelium tissues of SD rats after burn injury. Moreover,QHBDY can prevent effectively small intestinal epithelium cells apoptosis and decrease the damage of small intestinal epithelium tissues in the early stage of burning. Meanwhile,QHBDY can enhance expression of Bcl-2and inhibit the expression of Caspase-3in small intestinal epithelium tissues of scalded rats, which may be an important mechanism for QHBDY to guard against the apoptosis and protect intestinal mucus barrier.