TGF-β1,Smad3and Smad7in The Endometrium And Fibrosis Tissue Of Patients With Intrauterine Adhesions

Objective:This study was to investigate the expression of transforming growth factor-β1(TGF-β1),Smad3and Smad7in the fibrosis tissue and endometrium near the fibrosis tissue of patients with intrauterine adhesions(IUA), and to discuss the significance of these factors in the forming of IUA.Methods:The study group was composed by30patients with IUA and the control group included30infertility patients without intrauterine adhesions. We performed hysteroscopy check or surgery on all patients and got the fibrosis tissue and endometrium. Immunohistochemical method (streptavidin peroxidase conjugated method) was used to determine the expression of TGF-β1, Smad3and Smad7protein in these60cases.Results:1. The expression of TGF-β1, Smad3was higher in the fibrosis tissue and endometrium near the fibrosis in the study group than endometrium in the control group.(P<0.05)2. The expression of Smad7was lower in the fibrosis tissue and endometrium near the fibrosis in the study group than endometrium in the control group.(P<0.05)3. The expression of TGF-β1was positively correlated with that of Smad3in the study group (r=0.366、0.406p<0.05), but negatively correlated with that of Smad7(r=-0.388、-0.380p<0.05).Conclusions:1. The increased expression of TGF-β1of local uterine wall may be related to the occurrence of IUA.2. The abnormal expression of Smad3and Smad7of local uterine wall may be related to the occurrence of IUA.3. In the TGF-β1/Smad signaling transduction pathway,the excessive positive feedback of Smad3and insufficient negative feedback of Smad7may be related to the occurrence of IUA.