Monitoring And Analysis Of Pathogens Of Community-acquired Pneumonia In No.l Affiliated Hospital Of Anhui Medical University From2010to2011

ObjectivesTo analyze the variation of categories distribution with different seasons and age ofpatients in NO.1affiliated hospital of Anhui medical university from2010to2011, soas to provide a reference for clinical diagnosis and treatment.MethodsThis study included262adult with CAP who were admitted to Anhui medicaluniversity first affiliated hospital between July2010to June2011．Require patientsearly morning the first a deep well of the sputum, send the sputum bacterial separationtraining, fungi training. Patients with high fever were inspected blood culture.Bloodsample from patients were collected,extract serum.Using ELISA test to detect theatypical of the pathogen and virus IgM antibodies. Meanwhile, throat swabs formpatients were also collected. Using the polymerase chain reaction (PCR) technology todetect common6kinds of common respiratory virus, such as influenza A virus,influenza B virus, adenovirus, deputy influenza virus, respiratory syncytial virus,coronary virus, and atypical pathogens such as mycoplasma, chlamydia, corps bacteria,etc.ResultsQuinquagenarian were common in262patients with CAP in city Hefei. Men aremore than women. Through laboratory tests can be found, sputum cultures of positivedetection82plant pathogenic bacteria, including MRSA (~+)1strain, the detection rateis42.71%, and the G-bacteria accounted for51.22%, G~+coccus is occupied25.61%,the fungi was23.17%. The blood culture detection of pathogens in2cases, the detectionrate6.06%.262patients with CAP use of the serological ELISA method and the polymerase chain reaction (PCR) to detect atypical pathogens and respiratory commonvirus.35cases pathogens were detected by the method of ELISA, including the atypicalpathogens in15cases, respiratory virus20cases.43cases were detected by the methodof PCR, including the atypical pathogens in21cases, respiratory virus22cases. Themost common is mycoplasma pneumonia in the atypical pathogen,and influenza virus isthe most common in respiratory virus.ELISA method and polymerase chain reaction(PCR) method to detect the atypical pathogens and viruses have a certain difference, thedifference was statistically significant.There are certain seasonal differences inrespiratory virus infection, autumn and winter detection rate is higher.Double infectionwere9cases, accounting for3.44%of the total number. NOT found triple infection.ConclusionsThe existence of the inherent characteristics of hospital patients withcommunity-acquired pneumonia in the bacteriological bacteria to Gram-negative bacilli,and therefore, the need for the hospital community acquired pneumonia in the antibioticselection and prognostic assessment have a full understanding. Over the past year,hospital patients with community acquired pneumonia in addition to bacterial infection,there are still atypical pathogens, viruses and mixed infections, and of different agespathogen distribution, need to improve the atypical pathogens, respiratory viruses andmixed infections understanding. Although there are many pathogen detection methods,there are still some patients with CAP can not find the pathogens, and hence, how toimprove the specimen material way and testing methods to determine the pathogens tobe further investigated.