Clinical Significance Of EGFR And K-ras Gene Mutations And Protein Expression In The Lung Cancer

Background:Lung cancer as one of the highest incidence of malignant tumor in the world, and its incidence at0.5%of the annual rate of sustained growth, and advanced lung cancer5year survival rate still no clear increase (<15%). The development of lung cancer is the result of a variety of factors. There is important significance to understand of lung cancer occurrence and development of the relevant mechanisms, on the diagnosis and treatment of lung cancer.Abstract Objective:To investigate the differential protein expression and the gene mutation of EGFR and K-ras in tumor of the lung tissues and the relationship between the expression of protein and relevant clinical indexes.Methods:1. Different types of96cases of lung cancer and the surrounding normal lung tissue was collected. Immunohistochemical staining for EGFR and K-ras was performed in96cases of lung cancer and96cases of normal tissues.2. The selected of forzened tissue of non-small cell lung cancer (primarily for squamous cell carcinoma and adenocarcinoma) in31cases was measured using the RT-PCR assay in lung cancer tissues and normal lung tissues.The19th and gene21st codont mutations of EGFR and12th,13th and61st gene codon mutation of K-ras was measured.3. We consolidat the lung cancer clinical indicators (histological type, degree of differentiation, lymph node metastasis, gender, age et al), and statistic EGFR and K-ras gene mutation rate, EGFR and K-ras protein expression and the relationship among indexes.Results:1. In96cases of lung cancer tissues, it was high expression of EGFR in different types of lung cancer. Positive rate of EGFR in lung cancer (44.8%) was significantly higher than that in the normal tissues, and was related to histological types, lymph node metastasis, gender and age((P<0.05),and has no correlation with differentiation((P>0.05).2. It was high expression of K-ras in different types of lung cancer. Positive rate of K-ras in lung cancer (78.1%) was significantly higher than that in the normal tissues, and was related to histological types, lymph node metastasisage and age((P<0.05),and has no correlation with differentiation and gender (P>0.05).3. It was inverse correlation (P<0.05) between EGFR and K-ras expression in lung tissue.4. It was high expression of mutation of EGFR gene in different types of lung cancer. Positive rate of mutation of EGFR in lung cancer (35.5%) was significantly higher than that in the normal tissues, and was no related to lymph node metastasis, gender and age((P>0.05),and has correlation with differentiation((P<0.05).5. It was high expression of nutation fo K-ras gene in different types of lung cancer. Positive rate of mutioan of K-ras in lung cancer (20.9%) was significantly higher than that in the normal tissues, and was related to histological types, and gender ((P<0.05),and has no correlation with differentiation, age and lymph node metastasisage (P>0.05).6. It was no correlation (P>0.05) between protein expression of EGFR and mutation of EGFR gene in lung tissue. It was also no correlation (P>0.05) between protein expression of K-ras and mutation of K-ras gene in lung tissue.Conclusion:1. The overexpression of EGFR and K-ras were closely related to the formation and the development of the tumor of the lung.2. Mutations of EGFR, K-ras in genes involved in the pathogenesis of NSCLC.