| Polysaccharide from higher plants, animal cell membranes,microbial cells in natural substances, general polysaccharide often consists of more than one hundred or even thousands of monosaccharides by glycosidic linkages and. Polysaccharide is an important part of all living organisms, and necessary to sustain life for a variety of physiological functions of. Study found that the polysaccharide with antitumor, antiviral, antiinflammatory, hypoglycemic, anti-aging, anticoagulant, reducing blood sugar and other biological activity.Gentiana Gentiana for Gentianaceae, gentian, three flower gentian Gentiana rigescens Franch the dried root and rhizome. Experts and scholars at home and abroad has always been committed to gentian in chemical composition, pharmacological research.With petroleum ether reflux on medicinal gentian coarse powder skim, skim the medicinal material after the coarse powder by water extraction and alcohol precipitation method to extract. And then the seavge method of removing protein, in addition to protein polysaccharide were treated with95%ethanol, ethanol, acetone, ethyl ether and washing, is refined in Gentiana polysaccharides. The refined polysaccharides lowering blood lipid, anticoagulant and Study on antitumor effect of. Experiment study:1. Gentian plant polysaccharide on experimental liver injury in mice serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver tissue superoxide dismutase (SOD) and malondialdehyde (MDA) activity, to study the gentian plant polysaccharide hepatoprotective effect.2. Gentian plant polysaccharide on experimental hyperlipidemia rats, nutritional obesity mice, monosodium glutamate induced obese mice body weight, blood lipids, to study the gentian plant polysaccharide on reducing blood lipid function.3. Examines the gentian plant polysaccharide on mice thymus, spleen gland weight of influence; to mouse peritoneal injection of cyclophosphamide manufacturing model of immune suppression, carbon granule clearance method for determination of phagocytic function of mononuclear phagocyte, Gentiana polysaccharides on research in immunocompromised mice non-specific immune function; Determination of serum hemolysin, study of gentian polysaccharide on mice immune effect of specific immunity function.Experiment with gentian as raw material by using Chinese medicine polysaccharides classic extraction method of water extraction and alcohol precipitation method for extraction and purification of polysaccharides in Radix gentianae. And the extraction of the polysaccharides from the qualitative and quantitative analysis, the results show that, we extracted from the crystal is gentian of polysaccharides, and its content was206.42mg/g. Therefore, we may estimate the extracted raw material quality, and for large-scale extraction, for subsequent pharmacodynamic research ready. The experimentally observed gentian plant polysaccharide high, low dose group can significantly reduce the model of mouse serum alanine aminotransferase (ALT), aspartate aminotransferase activity (AST), and the results were statistically significant (P<0.01); polysaccharide high, low dose group can be significantly increased, the mouse model of liver tissue superoxide dismutase (SOD) activity, and the difference has statistical significance (P<0.01); polysaccharide high, medium, low dose group can significantly reduce the mouse model of liver tissue malondialdehyde (MDA) activity, and the difference has statistical significance (P<0.01). The above results indicate that gentian plant polysaccharide has certain protective action.Gentian plant polysaccharide can obviously reduce the experimental hyperlipidemia in rat serum TC, TG, LDL-C levels, increased HDL-C levels decreased significantly; nutritional obesity mice body weight and serum TC, TG, LDL-C levels, but on HDL-C levels are increased; can significantly reduce by monosodium glutamate induced obese mice body weight and serum TC, TG, LDL-C levels, increased HDL-C level, the above results suggest gentian plant polysaccharides with weight loss, hypolipidemic effect.Polysaccharide can be greatly increased in normal mouse thymus index and spleen index in normal mice, significantly improve the clearance index, enhance the phagocytic function of mononuclear phagocyte system, but also can increase the mice serum by half hemolytic value, showed that polysaccharide could enhance the immune function of mice with nonspecific and specific humoral immune function. Comparison of radiography and positive, polysaccharide on the specific humoral immunity and nonspecific immunity function in promoting the role and effect of Astragalus polysaccharide has no significant difference. |
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