| Electrospinning is a method to produce5～500nm diameter fibers byputting the high voltage electric field on a charged polymer solution forformation of the accelerated jet. Compared to the traditional spinningpreparations, the diameter of electrospinning prepared by this methodreduced1to2orders of magnitude. In medicine field, the drug-loaded fibersobtained by electrospinning method can effectively increase the elixirsurface area. In addition, the high porosity of electrospinning membrane(EM) could be favor of cell adhesion and proliferation, and its goodpermeability and moisture-penetrability could also provide goodenvironment for wound repair and healing. Therefore, EM has receivedeverybody’s widespread attention.In this study, polyvinyl alcohol (PVA) used as the carrier material is anon-toxic and non-irritating hydrophilic polymer containing a large numberof-OH groups. It can absorb a lot of water and form strong hydrogen bonds,and thus shows a good film. Sodium alginate (SA) is a non-toxic and controlled release material, which has a good biodegradability andcompatibility. The combination of SA and calcium ion has a very goodhemostatic effect, and thereby is widely used to hemostatic dressings andwound infiltration fluid dressings. In our study, using moxifloxacinhydrochloride (MH) as a model drug, we prepared MH/PVA/SA EM byelectrospinning method. According to pre-test results, solution spinnability,electrospinning morphology and speed were used as evaluation index forprescription screening. By the experiment, we confirmed that the bestprescription of MH/PVA/SA EM: the volume ratio of12%PVA and2%SAwas3:2, The MH drug loading of electrospun membrane was2%. And bythe preparation process optimization of electrostatic spinning vol tage，theflow velocity of the constant flow pump, distance between the nozzle andreceiver and spinning time, we finally choosed that the electrostaticspinning voltage was14kV, the flow velocity was0.3ml·h-1, the distancewas10cm and the spinning time was10h. Following, we preparedMH/PVA/SA EM patches, and then, the drug-loading and yield coefficientof MH/PVA/SA EM was also investigated. After studying of3batches ofsamples, we found that the load coefficient was (1.21±0.10)%and the yieldcoefficient was (86.73±1.83)%.In addition, in this study, a high performance liquid chromatography(HPLC) method was established and used for the determination of MHcontent in water and in PBS solution (pH7.4). By the method validation, we found that the method was accurate, sensitive and suitable for thedetermination of MH content in MH/PVA/SA EM and in vitro release ofthe EM. Then, drug release in vitro and the manner of the drug loading ofMH/PVA/SA EM were analysised. Through the fitting of drug releasecurve of MH/PVA/SA EM, we found that Higuchi equation had advantage.Combining with analysis results, we discovered that the manner of the drugloading of MH/PVA/SA EM was adsorption and embedding. Drug releasemechanism was dissolution, diffusion and corrosion. These studies maightprovide a theoretical basis for application of PVA/SA EM and in drugrelease.Besides, in this study, we explored the internal combination of MHand the carrier materials by using of microscopy, scanning electronmicroscope (SEM), and differential scanning calorimetry (DSC), infraredspectrum (IR) and ultraviolet chromatography (UV) analysis. Theexperiment demonstrated that MH dispersed in carriers in the form ofnon-crystalline.In order to study the stability of2%MH/PVA/SA EM patch, we usedspinning appearance, the drug crystal situation and the drug content in2%MH/PVA/SA EM as indicators, and conducted6months of accelerated testunder (40±2)℃and (75±5)%relative humidity (RH) condition. The resultshowed that2%MH/PVA/SA EM had good stability after3months.In addition, we investigated the permeability of MH/PVA/SA EM, the result showed that the EM had good permeability and met thecondition of good wound dressings.To explore the irritation of MH/PVA/SA EM,2%MH/PVA/SA EMpatches were used as treatment groups, PVA/SA EM patches as negativecontrol groups and sterile gauze as control groups. The skin irritation onrabbit damaged skin was investigated. The test demonstrated that thesegroups were almost non-irritating.For detection of bacteriostatic ability to S.aureus and E. coli, PVA/SAEM,0.5,2and4%MH/PVA/SA EM were used. The results showed that2,4%MH/PVA/SA EM had good antibacterial effect to S.aureus and E.coli.Furthermore, in this study, the back wound of SD rats was given2%MH/PVA/SA EM patche as a treatment group, PVA/SA EM patche as anegative control group and a commercially available woundplast as controlgroup, and wound healing pharmacodynamics of each group wasinvestigated. The result informed that2%MH/PVA/SA EM patches hadgood antibacterial properties and can promote wound healing. The resultsindicated that healing rate were64.98%and83.76%in3and8days aftermolding, and were higher than other groups. It showed that2%MH/PVA/SA EM patches can be used in the early stage of wound healing. |
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