Liver X Receptor Agonist GW3965Pretreatment On Rat Liver Transplantation Reperfusion Of The Liver Protective Effect Of The Experimental

Objective：The purpose is to research on the protective effect and thesystem after liver X receptor agonist GW3965preprocessing onischemia-reperfusion injury of rat’s graft liver.Methods: Separate Male SD(Sprague-Dawley)70rats into3groupswhich are sham operation group(SO group，14rats), Orthotopic livertransplantation group(OLT group,28rats),and GW3965preprocessinggroup(GW3965group28rats). Each groups operations were performedthrough semi open type ether inhalation anesthesia. For the SO group, Theabdominal cavity was dissected and separated into hepatoduodenalligament anatomy, While the other groups operations were conducted bydouble-oversleeve means for orthotopic liver transplantation. The levels ofserum transaminase, plasma inflammatory factors (TNF-α、IL-1), thechanges of hepatic pathology and inflammatory factor mRNA, and theactivities as well as its expressions of NF-κB in hepatic tissue wereobserved at6hour24hour after dissecting hepatoduodenal ligamentin SO group and after reperfusion of the portal vein in OLT group and GW 3965group．Results: after6hours24hours perfusion, the levels of plasmainflammatory factors (TNF-α、IL-1) is expression, serum transaminase, theliver pathological injury degree and the activities as well as itsexpressions of NF-κB in OLT group and GW3965group are higher thanthose in SO group. While after reperfusion for6hours and24hours, thelevels of serum transaminase, plasma inflammatory factors (TNF-α、IL-1)’expression, the liver pathological injury degree, inflammatory factor andthe activities as well as its expressions of NF-κB in GW3965group aremuch lower than those in OLT group； there are obviousdifference(p<0.05).Conclusions: After GW3965activation of liver X receptorpreprocessing, the activities of NF-κB and the emerging of downstreamInflammatory mediator factors are reduced effectively and protect the liverafter the ischemia reperfusion.