Study On MRNA Expression Of PXK In Peripheral Blood Leucocytes Of Sle Patients

Objection: To investigate the gene expression levels of PXK mRNA in peripheralblood leucocytes of SLE patients, the effects of PXK rs4075152polymorphism ontranscription level, and the relationship between mRNA expression of PXK andclinical manifestations of SLE.Method: According to the proposed standards, sixty SLE patients and thirty controlswere enrolled. Venous blood were collected and anticoagulated with EDTA. UsingRBC lysis solution to obtain peripheral blood leucocytes. Total RNA was obtainedusing Trizol and extraction kit. Reverse transcription-polymerase chain reactiontechnique was used to determine PXK mRNA expression of peripheral white bloodcells. Using SPSS13.0package to compare the difference of PXK mRNA expressionof peripheral blood Leucocytes between SLE group and control group, we analysedeffects of PXK rs4075152polymorphism on transcription level of mRNA, and thecorrelations between PXK mRNA expression and clinical manifestations.Result：1. PXK mRNA expressions of peripheral blood Leucocytes of SLE patientswere statistically higher than those of normal controls (t=3.295, p=0.001<0.05).2. No difference was found in SLE PXK mRNA expression between antinuclearantibody, anti-P0antibody, anti-u1RNP antibody, anti-Smith antibody, anti-dsDNAantibody, anti-histone antibody, anti-nucleosome antibody, anti-SSA/Ro60antibody,anti-SSA/Ro52antibody positive group and negative group (p>0.05). Expressionlevels of PXK mRNA was correlated with the production of the anti-Smithantibody(r=0.275,p=0.034<0.05), however no correlation was found between SLEPXK mRNA expression and the production of antinuclear antibody, anti-P0antibody,anti-u1RNP antibody, anti-dsDNA antibody, anti-histone antibody, anti-nucleosomeantibody, anti-SSA/Ro60antibody and anti-SSA/Ro52antibody (p>0.05).3. No difference was found between GG and AG genotypes in rs4075152of the SLE and control about PXK mRNA expression (p>0.05).4. No difference was found in SLE PXK mRNA expression between malar erythema,hypersensitivity to light, oral ulcers, arthritis, kidney damage, central lesions, thedamage of blood system positive group and negative group(p>0.05). No correlationwas found between SLE PXK mRNA expression and malar erythema,hypersensitivity to light, oral ulcers, arthritis, kidney damage, central lesions and thedamage of blood system (P>0.05).5. No difference was found in SLE PXK mRNA expression between ESR, CRP, IgG,IgA, C3, C4abnormal group and normal group (p>0.05). Expression of PXK mRNAwas correlated with the decreasion of C3（r=0.264,p=0.041）, C4（r=0.276,p=0.033）,however no correlation was found between SLE PXK mRNA expression and ESR,CRP, IgG and IgA (p>0.05).6. No difference was found in SLE PXK mRNA expression among SLEDAI score6-10group,11-20group and>20group (p>0.05). No correlation was found betweenSLE PXK mRNA expression and SLEDAI score (p>0.05).Conclusion:1.The expression of PXK mRNA is statistically higher in peripheralblood leucocytes of the SLE patients than that of normal controls;2. There are statistical correlation between SLE PXK mRNA expression and theproduction of anti-Smith, the decreasion of C3and C4;3. No relationship was found between PXK mRNA expression and rs4075152SNP.