Study On MRNA Expression Of TET3 In Peripheral Blood Mononuclear Cells Of SLE Patients

Background Systemic lupus erythematosus(SLE) is a chronic, relapsing, multi system autoimmune disease. The global prevalence rate of SLE is about 20-150/100000. SLE occurs in women, especially women of childbearing age, male to female ratio is 1:9. Autoimmune antibodies play an important role in the pathogenesis of systemic lupus erythematosus(SLE), clinical manifestations are diverse,including immune complex deposition in the kidney, brain, skin and inflammation of other organ systems. Genetic factors may increase the risk of progression of SLE. Genome-wide association analysies in the past few years have found that more than 55 Genetic susceptibility loci associated with SLE, such as IRF5, ITGAM, STAT4, MECP2, and so on.In 2012, based on the former GWAS studies of SLE, in order to discover more disease susceptibility loci / genes, and reveal the genetic pathogenesis of SLE, associated research group cooperate with the Hong Kong University research team of SLE analysis the GWAS data by meta analysis, and identited in mainland China and Hong Kong, China and Thailand three Asian independent populations, found five novel loci, rs34330(12p13,CDKN1B), rs6705628(2p13.1, tet3),rs6804441(3q13, CD80), rs4622329(12q23, DRAM1) and rs4948496(10q21, ARID5B),which were significantly associated with SLE, revealing the mechanism of cell cycle regulation, phagocytosis, DNA methylation and so on, which has an important role in the pathogenesis of SLE.Objective To investigate the different expression levels of TET3 in peripheral blood mononuclear cells(PBMCs) from SLE patients and healthy controls and the association between TET3 expression and SLEDAI or the clinical phenotype of SLE.Method We performed a RT-PCR in 89 patients with SLE and 87 controls. We extracted m RNAs from the peripheral mononuclear cells(PBMCs), and then reverse-transcribed into complementary DNA(c DNA), adding the corresponding fluorescent dyes and primers. Using ABI 7900 HT high-throughput fluorescence quantitative PCR Technology to detect the expression of TET3 in peripheral blood mononuclear cells. Analysis was performed with Statistical Package for the Social Science(SPSS) version 22.0. Data were expressed as mean ± standard deviation. P value < 0.05(two-tailed) was considered to be statistically significant.Results The expression levels of TET3 m RNA in SLE patients were increased compared with healthy controls’,also foud oral ulcer 、arthritis、 ds DNA、SM and SLEDAI associated with the expression levels of TET3. P value < 0.05(two-tailed) was considered to be statistically significant.Conclusion These results indicated that TET3 m RNA may be correlated with the pathogenesis of SLE.