| Purpose: To establish the rats model of experimental autoimmune myasthenia g ravis(Experimental autoimmune myasthenia gravis-EAMG) by emulsification liquid with AchRfragmentα1129-145and CFA. Observing EAMG rats serum IgG and IgM expression levelto explore the mechanisms and pathogenesis of MG by “warming yang and nourishing qi”Acupuncture and drug treatment method for EAMG rats. Further enrich the theoretical basisof Chinese medicine clinical acupuncture treatment of MG, and thus provides a new way forthe clinical treatment of “paralysis card”.Method:70female Lewis rats are choosed in this experiment, and randomly selected10rats to blank control group. The other60rats are used to establish The rats model ofexperimental autoimmune myasthenia gravis, respectively injected in the back, base of thetail, footpad according to the proportion of1:2with the emulsification liquid of AchRfragmentα1129-145and CFA. To observe the symptoms of myasthenia gravis in rats everyday, and decide whether the model is successful by the ways of gravis Lennon and theexpression level of AChR-ab titer. Then randomly selecting30rat and dividing rats intomodel group, acupuncture treatment group (“warming yang and nourishing qi” acupuncture)and drug treatment group (pyridostigmine),10rats in each group. blank control group andmodel group without any treatments under the same conditions; acupuncture treatment groupadopts normal reinforcement and normal reduction with needle and moxibustion one time for30min each day, and do continuously twe courses of the treatment which includes seven daysin one course, and between the courses to have one day of rest; the drug treatment group topour the pyridostigmine into stomach each day, do continuously15days. At the end of thetreatment, vein blood in the roots of all the rats tail were extracted and detected by ELISA inserum of IgG, IgM expression level.Result:①60rats to be established EAMG model after immunization for four weeksappear reduced activity, step-down action, weakened and lowers truggle, lower sounds anddarken hair symptoms; gravis symptoms gradually increase of rats after intensive immunation,and leading to3rats were dead because of serious gravis symptoms in the end. all AChR-abtiters shows P/N≥2.1which is according with the literature reported now days. the success rate of EAMG model is about51.7%.②IgG, IgM expression level in serummeasured by ELISA14days after the treatment showed that: the acupuncture treatment groupand the drug treatment group of EAMG rats serum IgG expression levels were significantlydecreased, acupuncture treatment group and the treatment group and model control groupcomparison, both has a significant sex difference (P <0.01), but the acupuncture groupefficacy compared with medical therapy group, no significant with sex differences (P>0.05);while the IgM expression level was no significant difference in each group(P>0.05).Conclusion:①The success rate of EAMG animal model is higher by acetylcholinereceptor (AChR)1129-145peptide fragments and complete Freund’s adjuvant (CFA)completely emulsified active immunization of rats.②IgG expression level in EAMG ratserum were significantly increased and IgM expression level is no significant changes, whichindicated that the changes of IgG expression level plays an important role in the pathogenesisof EAMG, and the close relationship is infered between IgG expression level and thepathogenesis of MG.③“warming yang and nourishing qi” Acupuncture can reduce theexpression level of IgG in the serum of EAMG rats, and thereby improving the symptoms ofmyasthenia gravis. That indicates “warming yang and nourishing qi” Acupuncture plays animportant role in the changes of IgG expression level in rats serum, and has good therapeuticfor MG in clinical. |
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