| ObjectiveTo investigate the isolation rates of Escherichia coli （E.coli）, the detection ratesof β-lactamases-producing E.coli, and the molecular epidemiology ofblaSHV-1-type gene of Dali,to explore the resistance of β-lactamases-producingE.coli and the infectious disease caused by them and to guide the use of antibioticsclinically.Methods1. Bacteria strains:213E.coli strains were collected from the MicrobiologyLaboratory of the Affiliated Hospital of Dali University, Dali prefecture Hospital andthe NO.1Hospital of Dali City.2. β-lactamase test: Test strip was used to determine whether strain producedβ-lactamase or not.3. Antimicrobial susceptibility test: The antimicrobial susceptibility tests wereconducted to all of the E.coli isolates producing SHV-1. The MICs of10antibioticswere determined and the drug-resistance of each strain was analyzed. Escherichia coliATCC25922, was used as quality control organisms according to CLSI guidelines.4. PCR amplification and molecular analysis. The plasmids ofallβ-lactamases-producing isolates were abstracted and subjected to the PCR amplification.5. Due to infectious diseases: access to medical records, were retrospectivelyanalyzed samples from bacteria and clinical disease. Refer to medical records, thebacteria were mostly isolated from sputum and urine and lead to infective diseases ofrespiratory system and urinary system. High incidence area was general surgicaldepartment, kidney internal department, urology surgical department and respiratoryinternal department.Results1. A total of202Escherichia coli isolates were collected, from three generalhospitals in Dali, including86from hospital A,74from hospital B,42fromhospital C.2. Of the202clinical E. Coli isolates, the E.coli strains producing β-lactamasewere167（82.67%）,including74from hospital A（86.05%）,57from hospital B（77.03%）,36from hospitalC（85.71%）.3. E.coli producing β-lactamase have higher drug-resistance rates to theantimicrobials,especially for ampicillin （100%）, cefuroxime （97.67%） andceftriaxone sodium（88.02%）,they are respectively while have lower drug-resistancerates to imipenem （0）,cefoperazone sulbactam （12.57%） and cefoxitin（15.57%）.colicefoperazone/sulbactam and cefoxitin could be used for theclinical treatment of E.coli producting β-lactamasethe, however, The drugs for thetreatment of the infection caused by β-lactamase-Producing strains should be chosenaccording to the result of the antimicrobial susceptibility test to avoid the side effectscaused by overuse of antibiotics.4. Of167β-lactamase-Producing E.coli,48strains were SHV-1-producingE.coli., the ratio was28.74%（48/167）. Including27from the Hospital A,12from theHospital B,9from the Hospital C.5. Refer to medical records, the bacteria were mostly isolated from sputum and urine and lead to infective diseases of respiratory system and urinary system. Highincidence area was general surgical department, kidney internal department, urologysurgical department and respiratory internal department.Conclusions1.Compared with the data from2010to2011, Producing β-lactamase bacteriaEscherichia coli resistance rates have increased year by year. Due to the extensive useof antibiotics and the emergence of multiple drug-resistant strains in clinical2The drug resistance of SHV-1-type β-lactamases-producing E.coli was serious.All of the β-lactamases-producing E.coli strains were susceptible to Imipenen, butthey showed different resistance to other antibiotics. Refer to medical records, thehospital stays of patients isolated with SHV-1-type β-lactamases-producing E.coli arelonger than the non β-lactamases-producing strains, considered to be related with thepathogenic of resistance gene needing further investigation.3. Of167β-lactamase-Producing E.coli,48strains were SHV-1-producingE.coli., the ratio was28.74%（48/167）. Including27from the Hospital of A,12fromthe Hospital of B21.05%（12/57）,9from the Hospital of C. SHV-1gene was foundamong three hospitals25%（9/36）By the result of PCR. |
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