The Study Of Radix Paeoniae Rubra Material Basis Of Efficacy On Liver Protection And Jaundice Retreat

The research and discovery of the new medicine and methods to treat icteric hepatitis has been always a focusing topic in the field of hepatopathy study. It has been approved through long-term clinical exploration that traditional Chinese herb medicines possess unique effects on liver disease treatment. Among those herbs, Radix Paeoniae Rubra (RPR) has gained extensive attentions by both Chinese and foreign drug research scholars, for its notable capacity in liver protection and jaundice retreat.In research and clinical studies, significant progresses have been made towards RPR’s efficacy in curing icteric hepatitis. However, the material basis of efficacy hasn’t been discovered, because the previous studies were mostly based on the effectiveness of single herb or its mixture. This study is trying to fill in this blank by targeting the study on Total Paeony Glycoside (TPG) and its major component paeoniflorin (PAE). Through utilizing the combinative methods of chemical separating ang pharmacodynamic tracking, the material basis of RPR on liver protection and jaundice retreat has been screened.The dissertation study contains two parts:literature review and experimental study.Part1:literature review.117research articles have been reviewed, analyzed and cited. The chemical composition of RPR, its extraction and separation technologies, and its pharmacology function were summarized. In addition to these, the research plan and design were also introduced in this part.Part2:experimental study. The research was conducted in the following5sub-categories.1、Preliminary screening of active site of RPR on liver protection and jaundice retreatFirstly, RPR slices were extracted using ethanol extract with70%alcohol to get alcohol extracting components. Then the rest dregs were dried and put in boiling water to get water extracting components. Later on, cholestatic jaundiced model of mice simulated by alpha-naphthylisothiocyanate (ANIT) and introduced as control group for the experiments. The efficacy of drug components between alcohol extraction and water extraction were tested. The results showed that the alcohol extraction was the active site.2、Constituent analyzing of alcohol extractiion of RPR and its components separatingThe HPLC method has been established in the study to determine the content of both PAE and ALB simultaneously, and PAE and ALB in RPR slices, alcohol extraction and water extraction of RPR were determined. The results showed that the PAE and ALB moved to alcohol extraction mostly. Technology of purifying TPG from alcohol extraction of RPR by macroporons resin was investigated with PAE and ALB as double indicators. Separate RPR alcohol extraction into sediment (component Ⅰ) and supernatant by dissolving in water, then separate the supernatant into TPG (component Ⅱ) and the remaining (component Ⅲ) by macroporons resin. PAE and ALB in these three components were determined. The results showed that the contents of PAE and ALB in component Ⅱ were as5.29and25.74times higher than those in component Ⅰ. There were little evidences in component Ⅲ.3、Active component screening from alcohol extraction of RPRSimilar to category1, cholestatic jaundiced model of mice was used again as control group to determine the efficacy of these components obtained in category2by using the mice serum biochemical indicators and the semi-quantitative analysis of pathological changes in liver tissue as indicators, and the pharmacodynamics units were screened. The results showed that comparing with the model group, the main indicators of component Ⅱ (TPG) and and component Ⅰ (sediment)-treated mice improved significantly, but the ones of component Ⅲ (the remaining)-treated mice showed no statistically significant.4、The preliminary study of liver protection and jaundice retreat mechanism of TPGThis part of experiment observed how TPG affects cholestatic jaundiced model mice’s sodium pentobarbital sleeping time, bile secretion, and liver uridine diphosphate glucuronic acid transferase (UDPG-T) activity. Results showed that sodium pentobarbital sleeping time of the mice were shortened in all three doses group of TPG. The bile secretion volume had obviously increased in the high and medium doses groups of TPG. The liver UDPG-T activity had improved in high dose group of TPG. The result suggested that the liver protection and jaundice retreat mechanism of TPG might relate to the increase of bile secretion, the enhancement of hepatic microsomal oxidese energy, the accrescence of liver’s detoxication, the improving of UDPG-T activity, and the promotion of bilirubin metabolism.5、The Study of PAE’s efficacy on liver protection and jaundice retreatObservation of how PAE affected serum biochemical markers and pathological changes on mouse model of cholestatic jaundice. The results showed that most serum markers of PAE’s three dose group were significantly lower than those in the model control group. As well, pathological changes in liver tissue of PAE-treated mice significantly improved. This suggested that PAE has a role in liver protection and jaundice retreat.