Research On Extraction And Isolation Of Radix Paeoniae Rubra And Intestinal Bacteria Transformation

[Objective]Research on preparation of paeoniflorin and albiflorin from Radix Paeoniae Rubra and transformation of albiflorin in vivo.We established the research foundation for the next step to study the effect of Radix Paeoniae Rubra as the substance of traditional Chinese medicine after oral administration.[Methods]Radix Paeoniae Rubra were extracted by ethanol water solution and then separated and purified repeatedly by column chromatography.The human intestinal flora was prepared by using albiflorin as the substrate of transformation.After the culture,the albiflorin was transformed in intestinal bacteria,then separated and purified the added points to prepare the product.[Results]A large amount of paeoniflorin and albiflorin were extracted from Radix Paeoniae Rubra,and their structures were identified.A human metabolite of albiflorin was isolated from the product,paeonilactoneA was isolated and identified by structure.[Conclusion]Paeoniflorin and albiflorin can be rapidly separated and purified by the purification methods and the transformation conditions.The albiflorin can be efficiently transformed into PaeonilactoneA.This method was not only suitable for laboratory research,but also for industrial production.It will be used in industrial production and will have a bright future.