Solanum Nigrum Alkaloids Myeloma Cell Apoptosis And Apoptosis Related Genes Of NF-κB

Solanum nigrum alkaloids myeloma cell apoptosis and apoptosis related genes of NF-kBObjective:This study of human multiple myeloma cell strains RPMI8226, to observe the role of Solanum nigrum of total alkaloids from two aspects:First, to observe the alkaloids of Solanum nigrum myeloma cell lines RPMI8226of pro-apoptotic role. Second, observe the effects of Solanum nigrum alkaloids on the expression of NF-kB, multiple myeloma, aimed at the molecular level initially revealed its anti-MM effect and mechanism of its in vivo anti-MM experiments provide an important experimental basis, developed with anti-MM effect of Chinese medicine drug preparation to establish the scientific basis.Methods:The human myeloma RPMI-8226cells were object of study, the totalal kaloids of Solanum nigmm test object RPMI8226cells, NF-kB is the evaluation of AnnexinV-FITC/PI flowcytometry of RPMI8226tumor cell apoptosis;points of NF-kB expression to explore the role of Solanum nigrum alkaloids myeloma RPMI-8226cells apoptosis and NF-kB in immunofluorescencc staining.Results:1. In the25mg/L of Solanum nigrum total alkali processing RPMI8226cells48h in RPMI8226cells significantly inhibited the growth 2. Solatium nigrum total alkaloids12.5-25mg/L concentration range, significantly inhibited the growth of myeloma cells in RPMI-8226, and gradually increased as theconcentration increased and prolonged inhibition.3. Drug concentration<25mg/L, induction of apoptosis is not obvious that the releaseof drug quality of50mg/L of early apoptosis increased significantly4. When the drugconcentration of25mg/L (obvious) the apoptosis, NF-kB expressionin a lower level of statistical significance.Conclusion:Solanum nigrum total alkaloids cytotoxicity in human multiple myeloma RPMI-8226cells were inhibit of RPMI-8226cells lines proliferation, promote cell apoptosis in a dose and time dependent. NF-kB expression levels in human multiple myeloma RPMI-8226cellline increases reduce the drug concentration with the total alkaloids o f Solanum nigrum.