Studies On Solanum Nigrum L. Protective Effects Against Liver Injury In Mice

Objective:With economic development, people living increasingly high, theincidence of liver disease has a rising trend. According to incomplete statistics,virulence hepatitis especially hepatitis B is the first in the world. The incidenceof fatty liver and alcoholic liver were also higher than before with theimprovement of living standards. Therefore, this is the urgent question thatseeking an effective drug treatment liver disease. Up to now, the developeddrugs especially chemical drug all have lots of disadvantage as poor selectivity,high prices, many shortcomings, et al. Such as: alpha-interferon, nucleosideanalogues, thymus peptide.Chinese patent medicine has lower toxicity, less side effects and wideresources, and special mechanism of action. In recent years, it was attented bymore and more researcher.“Longkuiguo”, the fruits of Solanum nigrum L., is a Uyghur medicine commonly used in Xinjiang of china. All over the country were distributed, wasborn in Tanabe, roadside or wasteland. Previous chemical investigations haveindicated the presence of alkaloid, saponin, carbohydrate and amino acid inSolanum nigrum L.. Pharmacological investigation demonstrated that it has theeffects of hepatoprotection, anti-tumor. In order to identify active site againsthepatic damage, the present study was to investigate the compound of Solanumnigrum L. and the effects on liver function.Methods:①108Kunming mice (SPF) were randomly divided into9groups, thenormal group, model group, positive drug group (200mg/ml), the95%ethanolextract of Solanum nigrum L. low-dose group (SNL-E-D, which is equivalentto the drug0.3g/kg), the95%ethanol extract of Solanum nigrum L. middle-dose group (SNL-E-Z, which is equivalent to the drug0.9g/kg), the95%ethanol extract of Solanum nigrum L. high-dose group (SNL-E-G, which isequivalent to the drug2.7g/kg), the water extract of Solanum nigrum L. low-dose group (SNL-W-D, which is equivalent to the drug0.3g/kg), the waterextract of Solanum nigrum L. middle-dose group (SNL-W-Z, which isequivalent to the drug0.9g/kg), the water extract of Solanum nigrum L. high-dose group (SNL–W-G, which is equivalent to the drug2.7g/kg).②I n addition to the normal group and model groupwere givenphysiological saline, the other groups were orally administration of7days. Onehour after the last administration, all groups were intraperitoneal injection with0.2%CCl4oleum olivae10ml/kg addition to the normal group, and forbid toprovid food but water;③In addition to the normal group, other groups were lavaged with 35°alcohol of16ml kg-1and the normal group was given distilled water after30min of daily administer. After giving alcohol on10th day, the mice wereforbided to provid food but water;④Take blood from ophthalmic vein after24hours in order to measure theserum aspartate aminotransferase (ALT) activity, aspartate aminotransferase(AST) and Synchronous Digital Hierarchy(SDH), reduced glutathione hormone(GSH), malondialdehyde (MDA) and superoxide dismutase (SOD)of liver. Takethe same parts from the liver tissue, fixed in formalin solution for testing.Results:The preliminary pharmacological experiment confirmed that thehepatoprotective effect activities of the ethanolic and water extracts of Solanumnigrum L. on carbon tetrachloride-induced and alcohol-induced hepatotoxicityin mice, it can reduce levels of serum aspartate transaminase(AST)(P＜0.05)alanine transaminase (ALT)(P＜0.05) and Synchronous Digital Hierarchy(SDH)(P＜0.05), increase reduced glutathione hormone (GSH)(P＜0.05) andsuperoxide dismutase (SOD)(P＜0.05), and reduce malondialdehyde (MDA)levels (P＜0.05)and ameliorate the histopathological changes fromCCl4-induced hepatotoxicity in the mice.Conclusion：The protect effects of the ethanolic extract of Solanum nigrum L. on carbontetrachloride-induced and alcohol-induced hepatotoxicity in mice was verylikely achieved by scavenging free radicals.