Effect Of Cinobufacini Combined With Radiotherapy On Proliferation And Apoptosis Of Human Gastric Carcinoma Cell Line BGC-823

Objective To investigate the effects of Cinobufacini(Cino) on the proliferation ofhuman gastric carcinoma cell line BGC-823. To analysis the impact of cell cycle andapoptosis of human gastric carcinoma cell BGC-823treated by Cino combined withradiotherapy. To explore the potential mechanisms of Cino combined with radiotherapy.Methods1.Growth inhibition rate of Cino in gastric carcinoma cell BGC-823atdifferent concentrations(0μg/ml,0.113μg/ml,0.225μg/ml,0.45μg/ml,0.9μg/ml,1.8μg/ml)and different time point(24h,48h,72h) was measured by MTT assay.2.BGC-823cellswere divided into four groups: control group, drug group, radiotherapy group, combinedgroup(Cino+radiotherapy). Cell cycles and apoptosis rate in different groups wereanalyzed by flow cytometry (FCM). The expression of cyclinD1and p16were detected byReverse transcription-polymerase chain reaction (RT-PCR).Results1.Cell growth and proliferation were significant inhibited in a concentration-and time-dependent manner.2.Compared with the control group, the proportion of cells inG0/G1phase were significant increased(P<0.05) and the proportion of cells in S phasewere significant decreased(P<0.05) in drug group, radiotherapy group and the combinedgroup. The apoptosis rates were also significant increased(P<0.05). Compared with druggroup and radiotherapy group, the differences were significant in the combinedgroup(P<0.05). Compared with the control group, the expressions of cyclinD1mRNA weresignificant inhibited(P<0.05) and the expressions of p16mRNA were significant increased(P<0.05) in drug group, radiotherapy group and the combined group. Compared with druggroup and radiotherapy group, the differences were significant in the combinedgroup(P<0.05). Conclusions1.Cell growth and proliferation were significant inhibited in aconcentration-and time-dependent manner.2.Cell cycle was arrested in G0/G1phase andapoptosis rate was increased treated by Cino combined with radiotherapy.3.The mechanismof synergistic antitumor effect of Cino combined with radiotherapy could be explained asfollows: the expression of cyclinD1was down-regulated and the expression of p16wasup-regulated in the combined group, which led to cell cycle arrested in G0/G1phase. Theapoptosis rate of BGC-823cells was increased.