Analysis Of Correlation Between Methylation Of Fas Gene And Expression Of Fas Protein And Fasl Protein In Tissues Of Esophageal Squamous Cell Carcinom

Objective： To investigate expression of Fas protein and FasL protein andmethylation of Fas gene in Esophageal squamous cell carcinoma tissues，Paracanceroustissues as well as in metastatic lymph nodes and non-metast atic lymph nodes. Analyze thecorrelation of Fas protein，FasL protein and methylation of Fas gene. Discuss the effectionthem may cause in development of Esophageal squamous cell carcinoma.Methods:54cases of esophageal squamous cell carcinoma tissues and correspond-ing paracancerous tissues were collected as well as35cases of metastatic lymph nodesand19cases of non-metastatic lymph nodes were collected from surgical patients.Fas andFasL protein expression of them were detected with immunohistochemical S-P method.Methylation of Fas gene of them were detected with methylation-specific PCR,MS-PCR.Analyze the correlation and difference among Fas protein，FasL protein，methylation of Fasgene and clinical-pathological data.Results：1.In tumor specimen the positive rate of Fas protein55.6%(30/54) is lowerthan85.2%(46/54) in corresponding paracancerous tissues (P<0.05). The positive rate ofdifferentiation of esophageal squamous cell carcinoma of Fas protein expression:highdifferentiation of84.2%(16/19),moderately differentiated of44.4%(12/27),25.0%(2/8) lowdifferentiation. Well-differentiated squamous cell carcinoma of the Fas-positive rate wassignificantly higher than in moderately differentiated or low differentiation (P<0.05).Different pathologic types of esophageal squamous cell carcinoma mdeulla, Tanumbrella,ulcers and narrowing between the various types of Fas protein positive expressionrate of59.3%(16/27),53.8%(7/13),50.0%(3/6) and50.0%(4/8)(P>0.05). Depth of invasion (T stage) of esophageal squamous cell Fas positive expression rate：T2of59.3%(16/27),T3of52.0%(13/25),T4of50.0%(1/2)(P>0.05);Lymph node metastasis in esophagealsquamous cell carcinoma of Fas protein positive expression rate was38.1%(8/21), withoutlymph node metastasis was66.7%(22/33)(P<0.05); Various clinical stages of esophagealsquamous cell carcinoma of Fas positive expression rate: Phase IIa61.9%(13/21), phaseIIb50.0%(5/10),phase III52.2%(12/23)(P>0.05).With no relations between sexuality,age(P>0.05).2. In tumor specimen the positive rate of FasL protein81.5%(44/54) is higherthan48.1%(26/54) in corresponding paracancerous tissues(P<0.05). FasL proteinexpression positive rate in esophageal squamous cell carcinoma of various degrees ofdifferentiation: well-differentiated57.9%(11/19),moderately92.6%(25/27),poorly differen-tiated100%(8/8), well-differentiated was lower than that in moderately or poorlydifferentiated(P<0.05); Different pathologic types of esophageal squamous cell carcinomamedulla, Tan umbrellas，ulcers and narrowing between the various types of FasL proteinpositive expression rate of81.5%(22/27),84.6%(11/13),83.3%(5/6) and75.0%(6/8)(P>0.05); Depth of invasion (T stage) in esophageal squamous cell carcinoma of FasL positiveexpression rate: T266.7%(18/27), T396.0%(24/25)，T4100%(2/2),T2is lower thanT3,T4period(P<0.05); Lymph node metastasis in patients with esophageal squamous cellFasL protein positive expression rate was100%(21/21), without lymph node metastasiswas69.7%(23/33)(P<0.05),clinical staging of esophageal squamous cell carcinoma ofFasL positive expression rate：phase IIa71.4%(15/21),IIb and90.0%(9/10)，III of87.0%(20/23)(P>0.05).With no relations between sexuality,age (P>0.05).3. In tumor specimenthe positive rate of methylation of Fas gene61.1%(33/54)is higher than22.2%(12/54) incorresponding paracancerous tissues (P<0.05). Different degree of differentiation ofesophageal squamous cell carcinoma of Fas gene methylation rate：well-differentiated63.2%(12/19),in the differentiation of59.3%(16/27),poorly differentiated62.5%(5/8)(P>0.05); the pathologic types of esophageal squamous cell carcinoma medulla，Tanumbrella，ulcers and narrowing between the various types of Fas gene methylation rateswere59.3%(16/27),61.5%(8/13),66.7%(4/6) and62.5%(5/8)(P>0.05);Depth of invasion (T stage)in esophageal squamous cell carcinoma of Fas gene methylation rate:T2of59.3%(16/27),T360.0%(15/25),T4100%(2/2)(P>0.05);Lymph node metastasis in patientswith esophageal squamous cell carcinoma of Fas gene methylation rate:61.9%(13/21),without lymph node metastasis:60.6%(20/33)(P>0.05)；The clinical staging ofesophageal squamous cell carcinoma of the Fas gene methylation rates were as follows：Phase IIa61.9%(13/21), phase IIb60.0%(6/10), III60.9%(14/23)(P>0.05).With norelations between sexuality,age(P>0.05).4. In lymph nodes Fas expression rate33.3%(7/21) is lower than that in non-metastatic lymph nodes66.7%(22/33)(P<0.05)；Metastasisgroup，lymph node FasL expression71.4%(15/21) higher than that in non-metastatic42.4%(14/33)(P<0.05);the transfer of lymph nodes Fas gene methylation rate57.1%(12/21)， higher than the non-metastatic lymph nodes24.2%(8/33)(P<0.05).5.Thecorrelation index(r) between Fas and FasL protein expression of the correlation is r=-0.33,P<0.05;Fas and Fas gene methylation of the correlation coefficient r=-0.56,P<0.05;FasL and the Fas gene methylation correlation r=-0.09, P>0.05.Conclusions：1. The expression of Fas protein in esophageal squamous cellcarcinoma is down-regulation，FasL up-regulation.the Fas gene is Hypermethylation.theFas and FasL expression was negatively correlated，Fas expression was negativelycorrelated with the Fas gene hypermethylation performance.2. In metastastic lymph nodesFas is down-regulation,FasL up-regulation,and Fas gene is hypermethylation.3.Fasexpression has relations with the degree of differentiation and lymph node metastasis.Inwell differentiated Fas expression is higher than in poorly differentiated(P<0.05)；in lymphnode metastasis Fas protein is lower than without lymph node metastasis(P<0.05). With norelations between sexuality,age pathological type，depth of invasion and clinical stage.4.FasL expression in esophageal squamous cell carcinoma has relations with the degree ofdifferentiation,lymph node metastasis,depth of invasion.well-differen tiated squamous cellcarcinoma of FasL expression is lower than in poorly differentiated(P<0.05);lymph nodemetastasis in esophageal squamous cell carcinoma of FasL protein was higher than thatwithout lymph node metastasis (P<0.05);T2period is less than T3,T4period(P<0.05). With no relations between sexuality，age，pathological type and clinical stage.5.The Fasgene methylation has no relations with sexuality,age,pathologic type, degree ofdifferentiation，depth of invasion，lymph node metastasis and clinical stage.