The Experimental Study Of Percutaneous Medical Ozone Injection On Transplanted VX2Rabbits

ObjectiveTo evaluate the safety and effectiveness of percutaneous medical ozone injection ontransplanted VX2rabbits.Method1. Model establishment: Twenty-four male New Zealand white rabbits （2．2～2．5Kg）were selected to establish the VX2carcinoma animal model which transplanted onto bothroots of thighs by percutaneously tumor suspension. Candidates of VX2models wereused2weeks later when the tumor diameter raised to1.6～3.4cm（2.3±0.85cm）.2. Grouping and treatment: Twenty-four carcinoma rabbits were selected in this studyand random divided into three groups：Group A：medical ozone concentration40μg/ml（n=9）；Group B: medical ozone concentration70μg/ml （n=9）; Group C：sham withouttreatment（n=6）. Rabbits in Group A and B were treated percutaneously with one shot of40μg/ml and70μg/ml medical ozone2ml respectively. Rabbits in Group C were operatedin the same method without injecting anything.3. Observation indicators：1) The general living conditions and complications were observed after the procedures.2) The serum ALT, AST and Cr were tested before the procedure and on the1thday、3^thday after the procedure.3) All the rabbits were killed in mercy3days post-procedure, then the tumor specimenswere extracted to observe the pathology change by HE staining method.4. SPSS13．0software was adopted to carry out the data processing and analyses： the measurement data were expressed asx±S and was put into test of homogeneity ofvariance before stastistics. One-way ANOVA analysis was deployed among all groupsincluding Bonferroni test. T Test was taken up for analyzing the group-in results.Categorical variable data was put into chi-square examination （P<0.05were significantdifferent）.Results1. The post-procedure rabbit’s living quality of each groupAll of the experimental animals in each group felt anorexy apparently. They were indepression, drowsiness and moved little but all recovered after24h. One animal in GroupAand B were dead within1hour respectively during or after the procedure. Both of themappeared with tachypnea，sobbing respiration and cyanosis and died after resuscitating therespiratory and circulating system by palpating the chest wall. It is speculated that therewas air embolus permeating into the vessel because early operate was not proficienctenough. A rabbit in Group B presented with limping temporarily. The dead rabbits wascomplemented timely.2. Liver function and renal function of each group2.1ALT: The serum ALT level pre-procedure in each group was A=52.89±22μ/L，B=56.67±31μ/L， C=59.17±24μ/L, variations of each group have no statisticallysignificance in each group（P＞0.05）. The1thday’s ALT level post-procedure in each groupwas A=72.78±24μ/L，B=69.56±28μ/L，C=67.00±29μ/L respectively and it elevatedapparently1thday’s after the procedure in each group and decreased from3^thday’spost-procedure to normal standard, which were A=54.78±20μ/L， B=57.22±29μ/L，C=62.67±21μ/L respectively. The differences of ALT level between3^thday post-procedureand1stday pre-procedure in all groups were not statistically significant（P＞0.05）. Thedifference of the3^thday’s ALT level between each group was not statistically significant（P＞0.05）.2.2AST: The AST level pre-procedure in each group was A=58.33±19，B=47.44±14，C=50.83±20respectively, variations of AST level pre-procedure in each group had no statistically significant （P＞0.05）. The1^th day’s AST level post-procedure wasA=68.33±16，B=88.77±39，C=62.83±32and it elevated apparently and decreased from3^thday’s post-procedure to normal standard, which were A=43.33±22， B=40.22±17，C=47.67±14respectively. The differences of ALT level between3^thday post-procedure and1stday pre-procedure in all groups were not statistically significant（P＞0.05）. Thedifference of the3^thday’s ALT level between each group was not statistically significant（P＞0.05）.2.3Cr: The Cr level pre-procedure in each group was A=52.60±29μmol/L，B=50.22±10μmol/L，C=50.00±10μmol/L respectively, variations of Cr level pre-procedurein each group had no statistically significant （P＞0.05）. The1^thday’s Cr levelpost-procedure in each group was A=78.56±32μmol/L， B=78.22±37μmol/L，C=51.33±14μmol/L respectively and it elevated apparently and decreased from3^thday’spost-procedure to normal standard, which were A=56.22±26μmol/L，B=57.89±18μmol/L，C=50.00±13μmol/L respectively. The differences of ALT level between3^thdaypost-procedure and1stday pre-procedure in all the groups were not statisticallysignificant（P＞0.05）. The difference of the3^thday’s ALT level between each group was notstatistically significant（P＞0.05）.3. Gross specimen and pathology observationNecrotic tissues in the neoplastic tissue and locally hyperemia and swelling wereobserved in Group A and B with naked eyes. In the ablative zone, liquefied nercrosis in thecenter surrounded by ivory chyle-like necrosis were also revealed. There was nostatistically different in Group A and B. Cylindrical fish-like tumor in rabbits cauld befound in Group C with naked eyes. Under the microscope, destruction of tissue structureand celluar morphology were shown in center of ablation zone along with degenerated,shrunken hepatocytes, pyknotic nuclei and lots of cell necrosis debris in both Group A andB. Clear line between tumor tissue and normal tissue cauldn’t be found instead of tumorinfiltration into muscular tissue, derangement and tumor cell nest in the tumor tissue inGroup C. Conclusion1. There are no harm to liver and renal function posed by medical ozone of40μg/m and70μg/ml.2. There are no significantly difference between both40μg/ml and70μg/ml group ingross specimens and pathological chip.3. It is safe and effective to percutaneously inject medical ozone in the solid neoplastictissue for local therapy of solid tumor under strict manual steps and real surveilliance.