Research On The Adhesion Of Silicate Bioactive Glass With Bacteria

The silicate glass research has made some progress, but for its antibacterial action mechanism has not yet formed the theoretical system, in particular to its associated with bacterial adsorption mechanism at present in China has not reported in literature system, relevant literature suggests,45S5antibacterial principle is through the contact damage bacterial cell walls, cause bacterial death, is on the tissue without damage to the specific bactericidal mechanism. So the study of its adsorption mechanism will be its antibacterial application key theoretical basis, the silicate bioactive glass research and application has great significance, with other materials and cells or histologic studies play an important role in the reference. This paper is to solve these problems to carry on the discussion, the antibacterial mechanism of bacteria adsorption mechanism mode of action, bacteriostatic topic further research provides theoretical foundation for its practical application, to provide theoretical support for.This paper mainly through the literature material law, the experimental research method, mathematical statistic method to observe and research of Staphylococcus aureus in45S5bioactive glass surface adsorption phenomenon, mainly through the study of biologically active materials45S5against Staphylococcus aureus and its adsorption mechanism of Staphylococcus aureus adhesion to form two aspects to form systematic theory. Experimental methods including in vitro using bacterial dynamic adsorption experiments, by environmental scanning electron microscopy, field emission electron microscope microscopic experiment means to explore its dynamic changes, by Gram staining,96hole plate biofilm detection, ESS optical density curve determination, isothermal adsorption curve determination,45S5charge adsorption experiments research of bioactive glass the essence and bacterial adsorption mechanism. The main results are as follows:1.45S5powder on bacterial adsorption significantly, small particle size, high concentration powder adsorption rate. 2.45S5powder and bacterial adsorption process is fast and physical adsorption, the adsorption isotherm adsorption equation to describe the available Langmiur. The adsorption pattern is typical of electric double layer adsorption, adsorption charge attraction for its main power.45S5powder can affect bacterial own biofilm formation, which was blocked by reversible adsorption phase, bacteria rely on adsorbed on the powder on the way mostly for bacterial deposition, Brown motion, the bacterial surface and protein adsorption layer (CB), electrostatic attraction between the liquid flow and other non-specific physical factors.