EGRRF And CK5/6Expression And Clinical Significance In Esophageal Squamous Cell Carcinoma

Background and PurposeEsophageal squamous cell carcinoma (ESCC) is the sixth most common cause of cancer death worldwide and80%of esophageal cancer occur in developing countries.Among them,90%are esophageal squamous cell carcinoma (ESCC).ESCC-related deaths is more than100/10000each year in the high incidence areas of China such as Linxian.Causing the high mortality is the lacking of precancerous lesions and early cancer diagnosis.So making clear the invasion and distant metastasis mechanism,as well as effective treatment, are still the main treatment for esophageal cancer at present. The domestic esophageal resection rate is90%.However, after5-year survival is only30%.Therefore,we have to learn the mechanism for early cancer diagnosis.In this study, we investigat the epidermal growth factor receptor (EGFR) and cytokeratin5/6(CK5/6) Then we analysis the expressions(EGFR, CK5/6) in the cancer tissue, adjacent tissues (within2mm beside the cancer tissue) and normal tissue.After that.we analysis the relationship among the expressions with patients’ age,gender,depth of invasion,lymph node metastasis and histological grade.AT last,we try to find the early diagnosis,the clinical treatment and prognosis for ESCC.Methods We randomly select60cases from the Nanjing General Hospital of Nanjing Military Region of cardiothoracic surgery from February2011to February2012after carefully reviewing the patient’s age, gender, medical history, endoscopy and pathology results, chest and abdomen CT surgical records and other information.Useing the immunohistochemical method to detect the60cases of ESCC cancer tissue and41cases of adjacent tissues, the expression of EGFR in42cases of normal tissue; Detected42cases of ESCC cancer tissue and normal tissue,41cases of adjacent noncancerous tissues of CK5/6expression. Explore the EGFR and of CK5/6expression among ESCC patients ages, gender, depth of invasion, lymph node metastasis and histological grade of the relationship between cancer and the organization of EGFR and CK5/6expression.Results1.60cases of ESCC cancer tissues,41cases and42cases of normal tissue beside the cancer of EGFR-positive expression were20cases (33.3%),3cases (7.3%), I patient (2.4%); among the statisticslearning differences (P<0.001).2.42cases of ESCC cancer tissue and normal tissue adjacent tissues,41cases of CK5/6positive expression were32cases (76.2%),17cases (32.7%),22cases (53.7%); There were significant differences between the three(P<0.001).3. ESCC cancer tissue, no significant differences between the expression of EGFR and the patient’s gender, degree of differentiation, lymph node metastasis and histological grade (P>0.05); but with age, a significant difference between the depth of invasion (P<0.05).4. ESCC tumor tissues of CK5/6expression was associated with gender, age, differentiation degree of the depth of invasion, lymph node metastasis and histological grade were not statistically different (P>0.05).5. ESCC cancer tissue EGFR and CK5/6expression, there was no statistically difference (P>0.05).ConclusionsⅠ. EGFR in ESCC cancer tissue, adjacent tissues, the expression in normal tissues showed a decreasing change.The ESCC cancer tissue expression have a relationship with patient age, tumor invasion depth.There is no clear association with the patient’s gender, degree of differentiation, lymph node metastasis and histological grade.2. CK5/6in ESCC cancer tissue, adjacent tissues and normal tissues showed a decreasing change.and there is no clear association with patient’s gender, age, degree of differentiation, depth of invasion, lymph node metastasis and histological grade.3. There is no a clear correlation between EGFR and CK5/6expression in ESCC cancer organization.