| [Background and objective]:The esophageal carcinoma is an one of the most familiar malignant tumor of our countries, it threatens seriously people life health. Cancerous occurrence in oesophagus development is tumor proliferation that the result that a many factors participates to turn into with many stages, its gradually, transfer but cause is an important factor to cause patient die. The research expresses that tumor start up invasion and metastasis because proteinase which is excreted by tumor cell separate Extracelluar Matrix. The connection of plasminogen's activation and tumor's invasion and metastasis is the most closely. Moreover, Cell adhesion molecules is also important to tumor's invasion and metastasis. Up to the present, there are very few studies of the correlation of uPA,P-selectin,p27 to the characters of clinical pathology of esophageal carcinoma, the study of the correlation of uPA, P- selectin, nm23, p27 in the esophageal squamous cell carcinoma is even more few. It provide a theoretic foundation for that unite examine uPA, P- selectin, nm23, p27 to find the meaning in invasion and metastasis of esophageal squamous cell carcinoma, in addition, it also tell us that restrain activation of uPA or P- selectin and increase activation of nm23, p27 is likely to a new therapy method of esophageal carcinoma.[ Materials and methods]: 54 cases of esophageal carcinoma were collected in tumor hospital of Anyang City Henan province. All tissues were identified by pathologist, it is entirely squamous carcinoma. 15 cases are I rank,30 cases are II rank, 9 cases are III rank. 21 example appeared the lymph node metastasis, each all in the site of cancer, cancer beside and far normal mucoma cut a piece of tissue. In situ hybridization and immunohistochemistry was used to analyze the expression of uPA, P- selectin, nm23 and p27 in normal esophageal mucoma, esophageal squamous cell carcinoma and atypical hyperplasia. Data were performed with SPSS 10.0 software. Chi-square test was used and statistically significant level was considered as "alpha equals 0.05".[Results]1. The positive staining of uPA mRNA in 54 cases esophageal squamous cell carcinoma was located at cytoplasm of neoplasm, it's expression had significant difference in histology classification, tumor size, invasion depth and lymph node metastasis group ( P<0.05 ) ; The positive staining of uPA protein in 54 cases esophageal squamous cell carcinoma was located at cytoplasm of neoplasm, it's expression had significant difference in invasion depth and lymph node metastasis group ( P<0.05 ) .2. The rates of uPA mRNA positive expression in esophageal squamous cell carcinoma, atypical hyperplasia, normal esophageal mucoma were 70.4%(38/54), 40.9%(9/22), 11.1% (6/54) , each other compared in three group had all significant difference (P<0.05 ) .3. The rates of uPA protein positive expression in esophageal squamous cell carcinoma, atypical hyperplasia, normal esophageal mucoma were 66.7% (36/54), 36.4%(8/22), 14.8%(8/54) , each other compared in three group had all significant difference ( P<0.05 ) .4. The positive staining of P- selectin mRNA in 54 cases esophageal squamous cell carcinoma was located at cytoplasm of neoplasm, it's expression had significant difference in histology classification, invasion depth and lymph node metastasis group(P<0.05) ; The positive staining of P- selectin protein in 54 cases esophagealsquamous cell carcinoma was located at cytoplasm and cytomembrane of neoplasm, it's expression had significant difference in invasion depth and lymph node metastasis group ( P<0.05 ) .5. The rates of P- selectin mRNA positive expression in esophageal squamous cell carcinoma, atypical hyperplasia, normal esophageal mucoma were 59.3% (32/54)31.8% (7/22), 9.3% ( 5/54) , each other compared in three group had all significant difference ( P<0.05 ) .6. The rates of P- selectin protein positive expression in esophageal squamous cell carcinoma, atypical hyperplasia, normal esophageal mucoma were 66.7% (36/54). 40.9... |
