Targeted Induction Antibody Agaisnt GPG And Proof Of Concept Of Motif Immunization

The development of an effective anti-HIV-1vaccine has been hampered by high mutation rates of the viral envelop, either within or outside of the epitope, among many other reasons. Using an innovative immunogen design, we generated a15-mer peptide that prominently expresses the triplet sequence GPG and induced high titer serum antibodies in mice and rabbits against GPG. Hybridoma was generated and a number of monoclonal antibodies were isolated. Immunological characterization of the antibodies demonstrated that the Mabs specifically recognized GPG and are capable of broadly neutralizing HIV-1isolates that carry the GPG sequence in the crown of V3loop, in a pseudovirus assay. Using the same design technology, conserved sequences derived from V2loop and gp41MPER also induced specific antibodies a that are not possible to induce using conventional immunogens; In addtion, we further explored the technology to induce antibodies to non-HIV sequences and demonstrated that this immungen design strategy allows us targeted induction of antibodies with desired specificities, even to arbitory epitopes.