| Objective:Analysis the impact change factors about peripheral blood circulating endothelial progenitor cells (EPCs) counts and vascular endothelial growth factor (VEGF), hypoxia-inducible factor-la(HIF-la) concentration in the west of high, middle and low latitudes healthy crowd. According to the elevation, nationality, age and gender factors analyzed and compared on the number of EPCs and peripheral blood of VEGF, HIF-1α factor concentration of healthy people. Described the number of EPCs, VEGF, HIF-1α factor concentration changes on peripheral blood of healthy people in the under conditions of elevation, nationality, age and gender factors provide a good research foundation for the prevention and control measures of various diseases related to the number of peripheral blood EPCs and VEGF, HIF-la factor concentration in the under conditions of elevation, nationality, age and gender factors.Methods:High altitude area in the northwest Gansu province GanNaZhou hezuo city in the Tibetan, altitude area in zhangye Sunan County Gansu province Tibetan, zhangye sunan yugu entered, AKeSai kazak jiuquan city,lanzhou han nationality of the gansu province, low altitude area XianYang city shaanxi province han residents stratified sampling method according to the health the select60cases samples and age is divided into20years of age,20to45years, more than46years old, each age group20cases samples were taken from each of the10cases the gender ratio. Each sample collection8ml peripheral blood crowd on an empty stomach,0.5ml whole blood using antibody surface markers and peripheral blood flow cytometric detection EPCs quantity, the remaining blood separation of plasma, using ELISA to detect the concentration of functional factors in the peripheral blood of VEGF, HIF-1α.Results:First, in accordance with the altitude factor the health of residents in the number of EPCs peripheral blood of VEGF, HIF-1α concentration:the conditions the same nation, age, gender factors, high altitude GanNaZhou hezuo city Tibetan higher than altitude sunan contry Tibetan residents; Altitude of Lanzhou city of Gansu Province Han rensidents higher than the low altitude Xianyang city of Shaanxi Province, the number of EPCs and peripheral blood of healthy Han residents in peripheral blood of VEGF and of HIF-1α concentration, the difference was statistically significant(P<0.05). Second, in accordance with the national factor relatively healthy residents the number of EPCs and peripheral blood of VEGF and HIF-1α concentration:The conditions of the same altitude, age, gender factors, elevation of Zhangye City Sunan Tibetan higher than Sunan health of residents. Zhangye city Sunan Tibetan higher than the Kazak health of residents; Zhangye city Sunan Tibetan higher than the Lanzhou city Han health residents; Jiuquan city Kazak higher than the peripheral blood of healthy residents of Lanzhou city Han residents; Sunan of Zhangye city is higher than Han health in Lanzhou city residents; Juquan city Kazak higher than the Sunan of Zhangye city. By comparison healthy residents between the age of peripheral blood EPCs number and VEGF, HIF-1α concentration:(A)high-altitude hezuo city Tibetan health residents in the conditions of the same gender factors. Three age stage health residents in number of circulating EPCs and VEGF and HIF-1α concentration comparison:20years of age is higher than the21-45year-old and46years of age Tibetan health21-45years old age than the more than46years old age Tibetan hezuo city (B)Sunan Tibetan health residents in the conditions of the same gender factors.three age stage health residents circulating EPCs counts and VEGF, HIF-1α concentration comparison:Age than20years of age21-45years old age of Zhangye city of Sunan Tibetan health residents; Age than20years of age more than46-year-old age of Zhangye city of Sunan Tibetan peripheral blood of healthy residents;21-45years old age above46years of age Zhangye city Sunan Tibetan health residents.(C)elevation of Zhangye City Sunan health residents in the conditions of the same gender factors. Three age stage health residents in peripheral blood the number of circulating EPCs and VEGF and HIF-la concentration comparison:Zhangye city Sunan health residents20-year-old age stage higher than the21-45age phase; Zhangye city Sunan health residents20years age age above46years age;21-45years old age than the number of EPCs in the peripheral blood of healthy residents the46-year-old age Zhangye city Sunan and VEGF, HIF-1α concentration, the difference was statistically significant(P<0.05).(D)Mid-elevation Jiuquan city Kazak healthy residents under the conditions of the same gender factors.three age stage health residents in peripheral blood the number of circulating EPCs and VEGF, HIF-la concentration comparison:20years age stage than21-45 age stage of Jiuquan city Kazak health of residents; Age above46years age in Jiuquan city Aksay Kazak health of residents;21-45years old age is higher than the46-year-old age stage in Jiuquan City, Aksay Kazak health residents in the number of EPCs and peripheral blood of VEGF and HIF-la concentration, the difference was statistically significant (P<0.05)(E) Mid-elevation, Lanzhou city of Gansu Province Han healthy residents.three age stage health residents in the number of circulating EPCs and peripheral blood of VEGF and of HIF-la concentration comparison:Lanzhou city Han20-year-old age stage higher than the21-45age phase health residents and age above46years old, healthy Han residents;21-45years old age is higher than46-year-old age Lanzhou City Han health residents.(F) low altitude Xianyang city of Shaanxi Province Han healthy residents three age stage health residents in peripheral blood the number of circulating EPCs and VEGF, HIF-1α concentration comparison:Xianyang city20years age stage than21-45years age stage Han residents;20years is higher than the Han healthy in Xianyang city age above46years old residents;21-45years old age than Han healthy in Xianyang City, age above46years old residents.Fourth, residents between the gender relatively healthy EPCs number in peripheral blood of VEGF, HIF-1α concentration:(A)Hezuo city high-altitude Tibetan health residents under the age of20,21-45years old, each age more than46-year-old male and female between the number of circulating EPCs in peripheral blood of VEGF and HIF-la concentration comparison, the differences were not statistically significance (P<0.05).(B) Mid-elevation Zhangye city Gansu Province Sunan Tibetan health residents under the age of20,21-to45-year-old, each age more than46-year-old male and female between the circulating EPCs number and peripheral blood of VEGF and of HIF-1α concentration, the difference was not statistically significant (P>0.05).(C)Elevation of Zhangye city of Gansu Province Sunan health residents under the age of20,21-to45-year-old, each age more than46-year-old male and female between the circulating EPCs number and peripheral blood VEGF, HIF-la concentration comparison was no significant difference (P>0.05).(D)Mid-elevation Jiuquan city of Gansu Province Kazak health residents under the age of20,21-45years old, each age more than46-year-old male and female between the number of circulating EPCs and peripheral blood of VEGF, HIF-1α concentration, difference was not statistically significant (P<0.05).(E)Mid-elevation Lanzhou city of Gansu Province Han healthy residents under the age of20,21-to45-year-old EPCs number of men and women between the gender of each age more than46years old and peripheral blood of VEGF and HIF-1α concentration comparison, the difference was statistically significant (P>0.05).(F) low altitude Xianyang city of Shaanxi Province Han healthy residents under the age of20,21-to45-year-old EPCs number of men and women between the gender of each age more than46years old and peripheral blood of VEGF and HIF-1α concentration comparison, the difference was statistically significant (P>0.05).Conclusions:The elevation, nationality, age factors produced impact on the hezuo city Tibetan health residents of Gansu Province in high altitude areas, Zhangye city Sunan Tibetan health residents of Gansu Province in mid-elevation areas, Zhangye city Sunan yugur health residents of Gansu Province. Jiuquan city Kazak health residents of Gansu Province, Lanzhou city han resident health residents of Gansu Province, Xianyang city Han healthy residents of Shaanxi Province the number of EPCs, VEGF and HIF-1α concentration in peripheral blood low-elevation, the difference was statistically significant (P<0.05). And gender factors on the number of EPCs, VEGF and HIF-1α concentration effect in peripheral blood healthy residents, the difference was not statistically significant (P>0.05). First, elevation of factors:(A)elevation of factors on the number of circulating EPCs in peripheral blood:With the elevation gradient increases, the number of circulating EPCs and peripheral blood VEGF and HIF-1α concentration upward trend.(B)elevation factors on the peripheral blood concentration of VEGF:Higher in high-altitude conditions the concentration of VEGF healthy residents than in the low altitude environment.(C) elevation of factors on the peripheral blood HIF-1α concentration:Conditions of high altitude factors higher than the health of residents in low altitude HIF-la concentrations. The peripheral blood of healthy residents in the same gender factors the number of circulating EPCs and VEGF, HIF-1α concentration have an impact. Third, the factors of age, nation the peripheral blood of healthy individuals of the same age the number of circulating EPCs and VEGF, HIF-1α concentration produced impact.Fourth, gender factors on the number of EPCs, VEGF and HIF-la concentration was not significant correlation in peripheral blood of the health residents. |
PDF Full Text Request