A Study Of Ginkgobalide B And Velvet Antler Polypeptide On Generation And Differentiation Of Cholinergic Progenitors

AD is a progressive neurodegenerative disease which is common in the old crowd,undergoing different disease stages with mild, moderate to severe cognitive decline.Thedisease may seriously influence the quality of life in patients’ families. The pathogenesis andpathological changes of neurodegenerative disease are complicated and various, mainlyresulting from neurons degeneration and death. None diseases of the CNS involving the lossof neural cells can be treated effectively by current therapies, which are predominantlysymptom relief approaches or strategies that aim at slowing down the progress, but can notstop the progression of the disease. Therefore, regenerative medicine will be a new therapyto complement lost neurons in the basis of preventing nerve injury. Numerous novel studiestoward the development of regenerative therapeutics should be accelerating.Today numerous researchers investigate NSCs and neuralizing factors, which haveproved that neural cells retain the capacity to generate new neurons. So regenerativetherapies have become a hot study which is benefit to diseases of the CNS involving loss ofneural cells. Research has been reported that the learning skills are better than before in theuse of AD mouse model after NSCs transplantation. However, whether neural replacementtherapy or regenerative therapies, the most important things is the differentiation of NSCs toavailably neurons.The experiment aim to find the effects of Ginkgobalide B and Velvet Antler Polypeptideon generation and differentiation of Cholinergic Progenitors, which will be benefit for theinvestigate of NSCs differentiation. To investigate the effects of GKB and VAP on inducingrat fetal’s telencephalon cells differentiation to cholinergic neurons is the essential objective,which is help for finding creative strategies to cure cholinergic neurons degenerative disease.The experiment is accomplished in Embryo Research Group of the Bethune jilinuniversity medical school. The telencephalon cells were plated equably into24well plateswhich was divided into four groups. The culture medium was added into suitableconcentration of GKB,VAP,both of the two and without any factor, respectively. Cell Culturewas terminated at4day, which were used to mark ChAT and Brdu positive cells byimmunohistochemistry. The positive cells were observed and counted using fluorescencemicroscope. The data of these positive cells were analyzed among each groups. We find that compare with control group, the neurospheres of experimental group were more bigger, andthe neurite were more and stronger, and more cells migrated.The number of ChAT positiveneurons in experimental group was more than that in the control group, especially the groupof GKB united with VAP, then the VAP group. The positive cells most distribute in theneurosphere, a small amount scattered among the neurospheres. The group of VAP has themost number of BrdU positive cells. Therefore, both GKB and VAP can induce neural stemcells to cholinergic neurons,combining both function more.