The Preliminary Studies Of The Differential Protein In The Chronic Prostatitis Patients EPS

Objective:By using protein electrophoresis technology and MALDI-TOF/MS technology, preliminary studying the different proteins in the prostate liquid between the chronic prostatitis patients and the normalMethods:Collect15samples in the type of chronic prostatitis and18samples of normal from the andrology department of The first hospital of Jilin university in the period2010.10to2011.09. Then we analyze composite samples by proteomics with follow steps:Protein electrophoresis Matrix-assisted laser desorption/ionization mass spectrometry-time of flight (MALDI-TOF-MS). Finally we statistically analyze the data by using relevant software and retrieval the proteins by using the protein database.Results:There were visibly five strips of the protein between the two groups in the result of the Protein electrophoresis. There were five proteins in the range of MW1000-12000Da between the two groups in the result of the MALDI-TOF-MS. The MW were3373,3483,4655,5322and10631, the first4of which were high expressed(P<0.001), the latter was low expressed (P<0.001). According to the results of the protein database, this five kinds of proteins may be:Brevinin-2Eg、Big endothelin-1、Alpha-defensin15、 Beta-defensin134and Prostatic steroid-binding protein.Conclusion:There were different proteins expressed in the prostate liquid between the chronic prostatitis patients and the normal men. Identified five differential expressions proteins. Brevinin-2Eg、Big endothelin-1Alpha-defensin15and Beta-defensin134in chronic prostatitis patients is higher than in the normal. Prostatic steroid-binding protein C2is lower. These proteins may be concerned with the chronic prostatitis...