The Clinical Study On Treatment Of Cerebral Stroke With Autologous Bone Marrow Stem Cell Transplantation

Objective:Confirm human bone marrow stem cell inducing differentiation to nervecell in vitro environment and discuss the clinical curative effect and safety ofautoallergic bone marrow stem cells treat cerebral stroke through subarachnoidcavity planting.Method:Our study contain two parts:First part: Separate and cultivate BMSCssuspension from fluid of autoallergic bone marrow of patients(patients havedone autoallergic BMSCs planting treatment),then send rapidly to ourlaboratory in hospital.After cultivating,amplifying and depurating in vitro,topassage in culture capsule,then detect and accredit surface marker throughmorphology feature and flow cytometry.After then,do inducing differentiationin vitro to bibasic cell that is passaged by tretinoin. Eventually accredit inducedcells by adopting.Second part:Select nineteen patients(including cerebral infarction andcerebral hemorrhage) who did autoallergic BMSCs planting treatment in thesecond neurology department in our hospital from August2008to December2011.Firstly,to do bone marrow aspiration with aseptic technique in operatingroom,then separate and cultivate in vitro to make BMSCs suspension,at lastinject the BMSCs suspension which is sepatated and cultivated in path ofLMP.Observe the clinical symptom before the autoallergic BMSCs plantingtreatment and follow-up visit after six months.Carry out appreciation forpatients’ change of neural function by adopting NIHSS score and BI exponent.Result: 1. It is detected that CD90and CD105of BMSCs’ surface antigen areexpressed by flow cytometry. Bibasic cell passaged in vitro whose marker isaccredited as BMSCs by carrying out flow cytometry.After inducingdifferentiation,apart cells express neural stem cell marker nidogen which alsocalled nestin.2. Compare clinical symptoms, objective sign and NIHSS score beforetreatment with after treatment of nineteen patients who do autoallergic bonemarrow stem cells treating cerebral stroke.Among them,ten patients improve indifferent degree on speaking and limbs myodynamia.Complication afteroperation:five among nineteen patients occur fever but after supplyingtreatment,all the patients’ temperature recover to normal in24hours,not occurother untoward reaction and complication.Conclusion:1.Human bone marrow stem cells amplify,cultivate and could induce toneuron type cell in vitro.2.There is no obvious complication of BMSCs treating cerebral stroke.It issafe and there is several curative effect,but it needs futher clinical observation.