| Object:To imitation the mechanism of human heroin readdiction and drug treatment, establishing the rat model of heroin readdiction and detoxification treatment, to study the changes in the capacity of learning and memory, and the changes of nitric oxide(NO), malondialdehyde(MDA), Superoxide dismutase(SOD) and the change of blood-brain barrier(BBB) permeability,to study heroin readdiction induced brain damage in pathological mechanism, and treatment for reference.Methods:①60SD rats were randomly divided into the readdiction group, detoxification groups and control groups (n=20).②Readdiction group established drug addiction'detoxification'readdiction to heroin relapse to addiction rat model. Detoxification group established drug addicts'detoxification relapse to detoxification rat model. Control group, the relapse group injection and fed with normal physiological saline. After the end of the modeling, the rats were given intraperitoneal injection of naloxone, assessment addiction strength.③onfirmed the change of the ability of learning and memory in the Morris water maze test after the successful modeling.④After the Morris water maze test,6rats were randomly selected in each group to directly decapitate and brains, the use of chemical colorimetric assay to test the change of NO, MDA and SOD in rat frontal lobe, parietal lobe, hippocampus, caudate putamen, diencephalon, cerebellum and brain stem7brain regions.⑤2rats randomly selected in esch group were observe the ultrastructural changes in rat brain BBB by transmission electron microscopy.⑥6rats extracted from each group were anesthetized, and brain tissue EB leakage observed by fluorescence microscopy.⑦6rats random from the each group, after anesthesia via the femoral vein to give EB, content of EB in brain tissue using fluorescence spectrophotometer.Results:①Compared with the control group and detoxification rats, readdiction group were significantly worse, naloxone experiment was positive (p<0.05);②The detoxification and control groups, readdiction rats escape latency (p<0.05); across the platform to reduce the number (p<0.05); time for the first time through the platform (p<0.05).③With detoxification and control groups, the relapse group NO content was significantly increased (p<0.05), MDA content was significantly higher (p<0.05), SOD activity was significantly decreased (p<0.05).④The electron microscopy showed that the relapse group and detoxification rats various brain regions of BBB series hyperpermeability ultrastructural changes.⑤With the control group and the detoxification group, relapse rats brain regions EB fluorescence spot number increased (p<0.05).⑥The compared with the control group and the detoxification group, relapse rats brain EB content increased (p<0.05), detoxification group than the control group EB content increased (p<0.05). Conclusion:①Subcutaneous injection of gradually increasing, in accordance with the addiction-detoxification-readdiction, heroin relapse model in rats, this modeling method is stable and effective;②Heroin readdiction to addiction group of rats to learn and memory ability, detoxification rats has recovered, but lower than the control group;③Heroin readdiction to addiction rat brain NO, MDA content increased, decreased SOD;④The heroin readdiction into addiction in rats and the other two group than in the brain BBB permeability increased;⑤Heroin readdiction into addiction in brain tissue of a free radical oxidative damage caused by the increase in BBB permeability, sparking a series of structural and functional changes of brain tissue, which may be heroin addicts brain damage a pathological mechanism;⑥Through effective detoxification of heroin readdiction into the addiction of learning and memory capacity will be restored, oxygen free radicals with enhanced. |
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