The Changes Of Oxidative Stress And The Energy Metabolism In Diabetic Nephropathy Rats

Objective Mainly to study the changes of oxidative stress and the energy metabolism in the diabetic nephropathy rats.Methods1seperat rats into two groups:randomly selected20Wistar male rats with220-250g of body weight, giving high fat high-energy food,normal control group give the ordinary food;2establish the animal model of diabetes mellitus and diabetic nephropathy:established the animal model through the high fat diet and a single small doses of STZ with celiac injection,normal control group give an injection of same amount of citric acid buffer.According to the urinary albumin/urine creatinine ratio up to30-300mg/g of the rat urine until28weeks,successfully established7animal model of diabetic nephropathy,deeming the experimental group(diabetic nephropathy group);3collect the specimen and process:after the diabetic nephropathy group and the normal control group anesthetized with10%chloralhydrate300mg/kg intraperitoneal injection,collecting the blood specimen of two groups,testing the biochemical indicators:blood sugar, blood urea nitrogen and blood creatinine;and take0.3g renal cortical of diabetic nephropathy group and normal control group;4test method:with immune method testing urinary albumin,with enzymatic determination testing urine creatinine,with chemical colorimetic testing the levels of MDA, SDH and ATP;5Statistical analysis With SPSS17.0analysis software, all results are expressed as means±standard error(se).The means values from each group are compared by t test.P value<0.05is used as the criterion for a statistically significant difference.Results1Blood biochemical indicator results showed that compared normal control group,the levels of BG has significant increased in diabetic nephropathy group(p<0.05).And the level of BUN and Cr between two groups has no significant difference(p>0.05).2Chemical colorimetry results showed that compared normal control group,the levels of MDA has significant higher while the activity of SDH and ATP has significant lower in diabetic nephropathy group(p<0.05).Conclusion1The levels of oxidative stress increased in diabetic nephropathy,showing oxidative stress has a close relationship with diabetic nephropathy;2The levels of energy metabolism decreased in diabetic nephropathy, tipping energy metabolism dysfunction may play a role in the pathogenesis of diabetic nephropathy.