The Molecular Spectroscopy Study And Application Of Some Drugs Based On The Ionic Liquid Extraction And Super-molecular Package Cooperation

To establish quickly, high precision and sensitive detectmethod is an important research direction in analysis chemistry.Despite many advanced instrumenttations have been used to detectanalytes in pharmaceutical preparations, biological specimens andenvironmental samples, the pre-processing-extraction of thesamples is indispensable. The analytes in complex samples can beseparated and concentrated through extraction. Hereby, the highsensitive and selective methods can be established. In thebackground of analytical chemistry and separation science, westudied the interactions between some drugs and ionic liquidthrough ultraviolet and fluorescence spectrophotometer, which wasin order to realize both the separation and concentration of analytesin complex samples and the establishment of new sensitivespectroscopy methods. There are four parts in this paper, and theconcrete research content and results are the following: 1. Reviewed the research of new“green solvent”-ionic liquid inbiological specimens, environmental samples and pharmaceuticalpreparations, and also the absorption and emission spectrum of themolecular package cooperation with CDP. Reviewed the absorptionand emission spectrum of the extraction effect of new ionic liquidand the research progress of fluorescence method of CDP in druganalysis.2. Ultraviolet spectrophotometer was used to investigate theinteractions between Mexiletine Hydrochloride（MXT） and sodium3,4-dioxonaphthalene-1-sulphonate（NQS）. An orange derivatizationwas obtained at pH 9.0, which can be extracted by ionic liquid. As aresult, the absorbency was obviously enhanced, while the maximumobsorption wavelength showed greatly red shift. Hereby, a highselectivity method （derivatization - ionic liquid extractionspectrophotometry） was established to determine MXT. Apparentmolar absorption coefficient of the extracted derivatives is2.69×10^-4L·mol^-1·cm^-1at 448nm. The concentration of MXT in therange of 0.2～5.4μg·mL^-1followed the Lambert-Beer law with aCorrelation coefficient of 0.9994 and detection limit 0.067μg·mL^-1.This method has been used to detect MXT in Pharmaceuticalpreparations and urine samples, while the recovery rates are 97.2%～100 % and 96.6 %～101.5 % respectively. 3. A newly, sensitive and high precision method（ionic liquidextraction spectrophotometry） was adopted to detect chelerythrinein water solution. The result showed that the fluorescence intensityof chelerythrine was obviously enhanced, and HMPF6showed thelargest increase. Thereby, a high sensitive fluorescence methodwas established to quantitative determine chelerythrine. Underoptimal conditions, the liner range is 0.2～130 ng mL 1, the detectlimit is 0.059 ng mL 1, concentration constant is 81.7 and theextraction rate of chelerythrine is 98.1％. This method has beensuccessfully used to detect chelerythrine in urine and bloodsamples.4. Fluorescence spectrophotometry was adopted to study theinteraction betweenβ–CDP and Palmatine chloride. One stableinclusion compound can be formed under strong base condition of6.0 mol l 1NaOH solution, while the mole ratio ofβ–CDP andPalmatine chloride is 1:1. The stability constant is 2.25×104l mol^-1which was measured by Double reciprocal plot. At the same time, adeeply and systematic research has been carried out to study themechanism. According to the special effect ofβ–CDP on Palmatinechloride’s fluorescence property, a high sensitive fluorescencespectrophotometer method was established to detect Palmatinechloride.