Experimental Study On Endovascular Repair After The Intravenous Catheter System Induced Phlebitis

Objectives:To observe and detect the repair of damaged veins which were given wet apply with50%magnesiumsulfate and fresh aloe respectively after setting indwelling needle as mimic real clinic transfusion to causephlebitis. To compare the treatment of phlebitis by using50%magnesium sulfate and fresh aloe, and toprovide the theory basis for curing phlebitis.Methods:Ear veins of healthy large-eared New Zealand rabbits were chose as the object of study which has twosteps.The first step: Establishing the model of phlebitis. Forty rabbits that had no edged-veins deformityand damage were chose to feed one week, their two ears edged-veins were chose as experimental vascular.Ten rabbits of these were chose as the control group randomly, the ears of which were bound up and fixedwithout indwelling needle catheter. Two edged-veins in both ears of other thirty rabbits were setintravenous indwelling needle as mimic real clinic transfusion,20ml of0.9%NaCl was infused in15minute, and then blocked with2ml25U/ml Heparin saline by positive pressure once daily. The ears of therabbits were wrapped by rabbits earcaps which were made by myself after infused to prevent the rabbitsfrom scratching them out. We disinfected heparin cap and pierced it by Syringe needle when infused again.Mechanical phlebitis was caused after indwelling needle catheter5days.The Second step: Performing Experiment by grouped. The indwelling needle was pulled out afterbuilding model successfully. The rabbits were divided into model group, experimental group I andexperimental group II, each group had10rabbits. The model group did not give any treatment. Afterintraperitoneal anesthesia, pathological sections of the model and the control group were prepared by taking1mm-long live samples of the ear vein puncturing at the0.5cm from the heart for transmission electronmicroscopy (TEM) observation and pathological sections were prepared by taking1cm-long and0.2cm-wide live samples from the ear vein which was obtained at1cm from puncture point for HE staining. Experimental group I was wet applied with50%magnesium sulfate; Experimental group II was wetapplied with Curacao fresh aloe which was cut into about5cm*3cm piece. This two groups were bound upwith plastic wrap and were wet applied2times a day, every time lasted4hours, and then live samples weretaken as above lasted5days. Specimen for TEM was put into4%glutaraldehyde fixative,4℃, acetonedehydrated, soaked, embedded, sectioned and stained after24hours, and specimen for HE staining was putinto4%formaldehyde fixative, ethanol dehydration, embedded in paraffin after24hours. Every specimenwas sliced3pieces of thickness2-5um continuous and the level of vascular inflammation was observed.Results:1. The degree of inflammatory reaction of model group was severer than that of the control group, Theinflammatory response difference had statistical significance（P＜0.01）.2. The degree of inflammatory reaction of Experimental group I was lighter than that of model group. Theinflammatory response difference had statistical significance（P＜0.01）.3. The degree of inflammatory reaction of experimental group II was lighter than that of model group,The inflammatory response difference had statistical significance（P＜0.01）.4. The degree of inflammatory reaction of experimental group II was lighter than that of experimentalgroup I. The inflammatory response difference had statistical significance（P＜0.05）.5. Observed in TEM, vascular vein endothelial cell surface of the control group had slightly protruding,mitochondria, rough endoplasmic reticulum and pinocytotic vesicles can be seen in Cytoplasm. Invascular cell of the model group, mitochondria were obviously swelling and vacuolar changes, roughendoplasmic reticulum were swelling and deformation and mild degranulation, slender processes andthe number of pinocytotic vesicles had reduced. In vascular endothelial cell of experimental group I,mitochondria were moderately swelling, rough endoplasmic reticulum were obviously swelling,particle fusion or degranulation could be seen. In vascular endothelial cell of experimental group II,mitochondria were slightly swelling rough endoplasmic reticulum were slightly expanded, and hadslightly particle fusion or degranulation.Conclusions:1. The stimulation of intravenous catheter system can cause phlebitis. 2.50%of magnesium sulfate and fresh aloe have the effect on phlebitis3. The treatment of phlebitis with fresh aloe is better than50%magnesium sulfate.