Inhibitory Effects Of Oxaliplatin And5Fu Slowly Released By PLA Ultrafine Fibers On HCT-8Cell Line

In recent years with the living standard of people and improve diet structure of the gradual change, the incidence of colorectal cancer also increased year by year, colorectal cancer is the serious harm human health life of malignant tumor, the treatment is mainly by surgical resection is given priority to, and complementary with chemotherapy, radiotherapy and immune therapy, etc. Comprehensive treatment. Chemotherapy treatment in an important role, and the traditional chemical treatment method for the whole body to medicine vein, although can to the tumor cells to produce some killer role, but the whole body drug use selective is poorer, bigger toxicity, side effects are many, many patients are difficult to tolerance. So, over the years, more and more research tend to avoid chemotherapy drugs body side effects and direct role in tumor local, including local antitumor drugs, including injection antitumor drugs slow-release implants, controlled release implants, and so on.With the development of science and technology, medical polymer widely application, make more the antitumor drugs may choose to become, and through the biodegradable polymer materials in can make combined, and to ensure the tumor within the scope of the place of blood drug concentration of lasting and stability of maintenance, which reduces the whole body of drug concentration, reduce systemic side effects.Based on this consideration, we and the Chinese academy of sciences changchun application research institute for cooperation, trying to find a safe and effective the biodegradable polymer drug release system, in the pack in patients after surgery of tumor growth area, in order to achieve the antitumor drugs slow release of purpose. This test is good biocompatibility, biodegradable polylactic acid (PLA) as a carrier material, the power spinning technology in medicine preparation for4%and20%of the rate of oxaliplatin into the carpet fiber and5fluorouracil implant to study medicine controlled-release dosage forms, proof contain mixed chemotherapy drugs of fiber felt slow release chemotherapy for colorectal cancer safety and effectiveness, which is expected to form the antitumor drugs to implant controlled release new dosage forms medicine, to succeed eventually used in colorectal cancer, ovarian cancer, pancreatic cancer, liver cancer and the clinical treatment of tumor.The results show that the drug release basic level with zero dynamics; Fiber felt group and pure medicine group compared to0.05P> no statistical significance, fiber felt compared with controls P <0.05a statistically significant flow cytometry, fiber art show that felt in the role of HCT-8of24,48,72,96hours, the tumor cells in G0/G1phase percentage significantly increased, and time was positively associated and S period, G2/M percentage of relatively less, and time was negatively correlated. Conclusion:carry oxaliplatin into and5fluorouracil fiber felt has obvious effect of slow release, in the role of colon cancer cells in vitro people HCT-8can effectively restrain cancer cell proliferation, stop the cell cycle process.