Effect Of A Bleaching Agent And Fluoride On The Adherence Of Oral Bacteria To Human Dental Enamel Within24Hours

[Objective]To evaluate the effect of a bleaching agent and fluoride on the adherence of oral bacteria to human dental enamel within24hours.[Methods]35enamel specimens, prepared from seven human third molars, were divided into five groups with different treatments. Group A:Bleaching+Fluoride+LED Light; Group B: Bleaching+LED Light; Group C:Fluoride+LED Light; Group D:Saline+LED Light; Group E:Saline without LED Light. After36min treatment, specimens were disinfected and embedded in human oral cavity for30min,1h,2h,4h,8h,12h and24h. Enamel morphology and bacteria photos were taken under SEM for further analysis.[Results]Magnify by5000, within4h, all groups had no significantly different, and the numbers of bacteria were less than30. But from8h to24h, Group B showed a significantly higher number than other groups (p<0.01). Magnify by2000, Group B showed the higher roughness than other groups (p<0.01), the rest groups showed no significant different from each other (p>0.05). [Conclusion]Based on the results of the present study, tooth bleaching roughed tooth enamel and accelerated the adherence of oral bacteria to human dental enamel, while fluoride inhibited this effect.