The Study Of IL-17and IFN-γ In New Diagnosed Type2Diabetes Mellitus Patients

Diabetes is a chronic and hyperglycemic metabolic diseases.Theprevalence of diabetes is increasing with the aging of population,the llivingstandard’s improving, the change of living style and living enviroment.Moreand more evidence reveals that natual and adaptive immune systems may allinvolve in the occurrence and development of diabetes. The researches onadaptive immunity of diabetes are much more than natual immunity. Type1diabetes is an autoimmune and progressive destruction of pancreatic β cellswhich is mediated by the T cell, and many immune cells and inflammatorycytokines involved in the occurrence of type1diabetes. Many studies haveshown that the helper T cells and their inflammatory cytokines participate inthe pathogenesis of type1diabetes.The immune factors in the occurrence anddevelopment of type2diabetes are paid much more attention recent years.Th17and Th1cells and the inflammatory cytokines IL-17and IFN-γ theyproduced are also the hot pints nowadays.Th1cell and IFN-γ have beenproved involved in the type1diabetes.However,as to the type2diabetes,particularly the new diagnosed type2diabetes mellitus,there are few reportson this issue. The researches about the relationship between Th17cells andthe diabetes are increasing, but the pathogenes is still a inconsonant conclusion.At the same time, Th17and Thl cell may exist in the close contact in thedifferentiation, so the Th1and Th17cells in type2diabetes is not clear.Theobject s of this study are recently diagnosed type2diabetes withouttreatment,and we collect the blood sample of these objects and detect thefrequency of IL-17and IFN-γ.Our aim is to explore whether the CD4+T cells are involved in the type2diabetes,and look for new targets for the type2diabetes prevention and treatment.PurPose:To observe the IL-17and IFN-γ in the recently diagnosed type2diabetes, and to explore whether the CD4+T cells are involved in the type2diabetes.Materials and Methods:16recently diagnosed type2diabetes without treatment and9normal controls were selected. All the objects were done fasting bloodcollection in the early morning.All the patients’s blood were done PBMCseparatiion,and then IFN-γ, and IL-17cytokines were detected by FACS.Result:Compare to control group,the IL-17is significantly higher in therecently diagnosed type2diabetes (P<0.05),but IFN-γ is not significantlydifferent in the recently diagnosed type2diabetes compare to controlgroup(P>0.05).