The Study On Effect Of Low Level Laser On Artificial Bone Healing In Vivro

Oral implant restoration has now become a routine repair of dentitiondefects/missing, but because of missing teeth and a long time, the bonecondition was poor, frequently resulting in the growing areas of bone massdeficiencies. Bone grafting is used to solve this problem. However, aftertreatment by bone grafting, and often require period of6to12months for bonehealing, making the growth of the implant treatment regimen and a lot ofinconvenience for patients;The bone quality is an important factor to affect thesubsequent planting success rate, so how to accelerate the speed and quality ofosteogenesis as a clinical bone graft problems is to be solved. Low-energy laser,its wavelength range is about600~950nm, output power is less than250mW,with the effect of biostimulation, which can be generated to reduceinflammation, and promote the role of tissue repair,.It is a non-invasive,non-contact treatment, it has a lower output energy, the irradiating tissuetemperatureand will not be higher than the normal tissue causing tissueinjury.The studies have shown that: the low-energy laser has function ofpromoting bone repair, which is an important positive role of osteogenic cellsand fracture healing.Despite the existence of a large number of studies have shown thatlow-energy laser can be used to repair of bone formation, but the specificmechanisms of its role and laser dose selection are not understanding, whichneeded more and better experimental studies to be determined andsupplementary. Given the role of low-energy laser on bone repair, it is greatsignificance, which may strengthen peri-implant bone healing, improve timeand quality,planting success rate of bone graft, improve.In this study, theperspective of in vitro experiments,which verified LLL promoting osteoblast- like cells MG-63proliferation, VEGF secretion in the MG-63and artificialbone co-culture system,that provides a theoretical basis for LLLT used in thefield of Oral Implantology.1.Different doses of low-energy laser irradiation on the MG-63value-addedObjective: To verify whether the dose of LLL can promoteosteoblast-like cells MG-63proliferation in vitro, and which is more effective.Method: The MG-63osteoblast-like cells were cultured in high glucoseDMEM medium containing10%fetal bovine serum. The experiments wererandomly divided into experimental group (L) and the control group (C), Lgroup,the laser group, C group without irradiation. Experimental group isdivided into4group (L1, L2, L3, and L4), L1radiation energy density1.25J/cm2L, L2is2.5J/cm2, L33.75J/cm2, L4irradiation energy density of5J/cm2. Irradiation way for the average daily irradiation energy density1.25J/cm2.5days, supernatants were collected to frozen stand; MTT assay cellproliferation.Results: The energy density of no effect is1.25J/cm2in the cellproliferation; energy density of2.5J/cm2,3.75J/cm2,5J/cm2promote humanosteosarcoma cell line MG-63proliferation;5J/cm2, the low-energy laser is themost obvious role in promoting human osteosarcoma cell proliferation.2.Observational study of low-energy laser on the osteogenic properties ofthe MG-63cells with artificial co-culture system.Objective: different doses of LLLT is effective in promoting bone-likecells and artificial binding ability, thus affecting the time and quality of bonerepair.METHODS: Experiments were randomly divided into two groups:experimental group (L), the control group (C), L is the group for the laserirradiation group, depending on the radiation dose is divided into four group (L1, L2, L3, L4), the nano-hydroxyapatite into centrifuge tubes, centrifuged,and to join the high sugar and high glucose DMEM medium, the bone iscompletely immersed in the culture medium. Set the high-speed centrifuge,centrifuge,12000rev/min,5minutes. Bone placed in24well plates, andhuman osteoblast-like cells MG-63with a density of1.0×104/well wereseeded in24-well culture plate and bone,24hours after the medium waschanged, laser irradiation, dose and the same way as the experimental group C2and the other to a culture plate as a control group does not effect. Day5supernatants were collected stand; scanning electron microscope bone and cellgrowth.Results: The energy density of2.5J/cm2,3.75J/cm2,5J/cm2, thelow-energy laser irradiation can promote the human osteosarcoma cell lineMG-63in the proliferation of artificial bone material (nHAC/PLA) surface,and various energy the density of low-energy laser can promote the growth andadhesion of the surface of cells in the bone material.3.Different doses of low-energy laser on the MG-63cells secrete VEGFObjective: In vitro studies of different dose LLL irradiation on the MG-63with artificial bone material (the nHAC/PLA) hybrid system, the MG-63secretion of VEGF.Methods: Experiment1, the cell culture supernatant and experimentaldetection of artificial bone co-culture system with human osteoblast-like cellsMG-63supernatant VEGF factor for ELISA method.RESULTS: the energy density of1.25,2.5J/cm2, promote humanosteosarcoma cells MG-63with artificial bone co-culture system, the MG-63cells expression of VEGF was promoted strongly when irradiation dose is1.25,2.5J/cm2; energy density of3.75J/cm2has no effect on the MG-63,VEGF secretion; energy density of5J/cm2on the MG-63of VEGF secretioninhibited. Conclusion:2.5J/cm2, the application of LLLT on cell proliferation andsecretion of VEGF were promoted, while promoting the adhesion andproliferation of the surface of cells in the bone substitute material is to promotethe ideal artificial bone into bone The radiation dose of LLL used in the oralcavity after implantation of bone healing and reconstruction, and may shortenthe time of bone formation and improve the quality of bone, thereby reducingthe implant restoration treatment. In this study, provides a theoretical basis forLLL used in oral implant clinical, to provide a new method for tissueengineering of bone vascularization.