Cytokines On The Impact Of The Broken Bone Resorption And Osteoblast Proliferation And Into The Bone Function In Vitro

Mandibular desease is one of the commen desease in oral maxillofacial surgery. Mandibular function disorders caused by abnormal bone resorption and bone defection compose the main pathological changes. The methods of isolating and culturing osteoclasts and osteoblasts were established in this study to provide substaintial experiment basis for further research of the physiology and pathology of bone metabolism, induction of osteogenetic activities of bone growth factors, as well as in vitro research of bone graft and dental implant materials.1. Isolation and culture of osteoclasts: Osteoclasts were isolated and cultured from long bone cavities of human aborticide fetus. The observation with histomorphology and phase contrast microscope: osteoclasts were large in size, multinucleus, foam-like cytoplasm with vacuole and pseudopodia, and movable. Acid Phosphatase reaction is positive. The cells were cultured with human femaral cortical bone slices, thereafter the absorbed pits appeared on bone slices. The results showed that the methods is stable and practical.2. Isolation and culture of osteoblasts: Osteoblasts were isolated and cultured from craniobone of neonatal rabbits with modified enzyme digestion method. In addition of cytomorphologic identification, immunolhistochemic technique of bone morphogenic protein-monoclonal antibody (BMP-McAb), alkaline Phosphatase staining and concentration assays, as well as osteoblasts reaction to PTH-cAMP were used, according to the biologic character of osteoblasts. The results proved that the osteoblasts from neonatal rabbits retain their function and character, which can be used for experiments.