Changing Cystatin C During Rabbit Orthodontic Tooth Movement And Molecular Biological Mechanism Of Periodontal Remodeling

Orthodontic is progressing in 21th century.How the tooth can move stablyand efficiently in best times has always been a hotspot.Shorten the course oftreatment,found the most suitable force and the indicator to detect the effect oforthodontic in order to found out the method of improving the speed is veryimportant.Therefore,I will do a research on this field.Objective:In this paper we study the change of Cystatin C during rabbitorthodontic tooth movement and its characteristics with orthodontic forceapplied at different times, and investigate the molecular biological mechanismof periodontal remodeling.Methods:An experimental model of orthodontic tooth movement wasestablished in 40 Japanese Ear Rabbit with eight of about 2.0kg, which weresacrificed at 1,3,5,7, and 14 days after application of about 80N orthodonticforce. The gingival crevicular fluid was extracted and the content of Cystatin Cwas calculated using ELISA. The morphological changes were observed viaHE staining. The Cystatin C in the bone was quantified with SABC.Results:A remarkable decrease in Cystatin C was found at the beginning stageof the tooth movement while osteoclast has increased. On the other hand, thecystatin C increased and osteoclast decreased towards the end of themovement.Conclusions:It is concluded that Cystatin C participates in proteolysis duringorthodontic tooth movement, and it changes with the time of orthodontic forceapplication.