Relevance Between Substance P And Focal Adhesion Kinase, Protein Kinase B, And Nuclear Factor κ B In Scar Formation

Objective:And in a proliferation of scar fiber cell proliferation as the main。In the previous study confirmed FAK、PKB、NF-κB protein in proliferative scar the expression in the increased significantly。So we speculate that the exogenous substance P likely, as a kind of important factors to activate all kinds of signal transduction pathways has an important role。This experiment discusses different concentrations of exogenous substance P into fiber cell proliferation influence and exogenous substance P and gelling spot kinase, protein kinase B, nuclear factor in the relevance and scarring timeliness,。For future clinical treatment aims to provide the referenceMethods:1. The experimental method:Choose CCC-ESF-1human embryonic fibroblasts for regular training, take the logarithm of cell lines of different cells SP concentration intervention, The method of observation MTT SP into fiber cell active influence, on the enzyme league immune detector determine the optical density, Through the cell count, draw cell growth curve to explore SP fibroblasts proliferation influence, The concentration of SP1×10-6mol/1solution stimulate fibroblasts; With Western blotting detection fibroblasts FAK protein, PKB proteins, as well as the NF-κB protein expression.2. Statistical methods:MTT Experimental results with average and standard deviation to said, Western-blot experiment each index of income data mean±standard deviation with (x±s) said。The statistical data between groups with single factor analysis of variance (ANOVA) test. The above process are SPSS13.00use statistical software processing. P0.05for with a statistical significance.Results:1. MTT Results: In different concentrations into fiber cell proliferation SP stimulation, Dose1×10-8mol/1to1×10-5mol/1along with the change of concentration。With the change of concentration, each group and control group than a significant statistically significant, Tip SP promote into fiber cell proliferation has certain concentration dependence, With increased concentration is SP, promote fibroblasts proliferation the stronger ability.2. Western-blot results:The results show When the1×10-6mol/1SP role in fibroblasts0min, FAK、PKB、NF-κB with a small amount of the activity, When15min increased level of activity, to30min activation when most obvious, peak, and then gradually declined, and last at least60min. And in different time points difference expression with a statistical significance。(F=8.259, P<0.001; F=36.674,P<0.001; F=41.472,P<0.001)。1) SP stimulation also by some in the activation of the HS Fb FAK level:FAK relative grey value compared with0min,15min,30min,60min than0min respectively up39.2%(P<0.05),73.9%(P<0.05) and38.5%(P<0.05)。30min compared with15min, the expression level was24.9%rise significantly (P<0.05),60min compared with30min down20.3%(P<0.05), The expression level have obvious change, the difference was statistically significant (P<0.05).2) SP stimulation also by some in the activation of the HS Fb PKB level:PKB relative grey value compared with0min,15min,30min,60min than0min respectively up29.0%(P<0.05),76.5%(P<0.05) and44.7%(P<0.05)。30min compared with15min, the expression level was36.8%rise significantly (P<0.05),60min compared with30min down18.0%(P<0.05), The expression level have obvious change, the difference was statistically significant (P<0.05).3) SP stimulation also by some in the activation of the HS Fb NF-κB level:NF-κB relative grey value compared with0min,15min,30min,60min than0min respectively up44.5%(P<0.05),78.3%(P<0.05) and0.09%(P<0.05)。30min compared with15min, the expression level was23.4%rise significantly P<0.05),60min compared with30min down38.9%(P<0.05), The expression level have obvious change, the difference was statistically significant (P<0.05). Conclusion:1. Substance P can promote wound healing and scarring2. Substance P can promote into fiber cell proliferation, and have certain concentration dependence, that is, the higher the concentration with SP, promote fibroblasts proliferation the stronger ability.3. Substance P role and fibroblasts, and a short time FAK activation, PKB, the nf-kappa B protein relevant.4. Substance P promote into fiber cell proliferation may and integrin mediated FAK way, phosphatedyl inositol232kinase (phosphatedyl inositol232kinase, PI3K) way, nuclear factor relevant way.