Preparation Technique,characterization And Biological Effects Of Nanoparticles As BFGF-P(HB-HO)

Temporal control of bioactive signals in tissue engineering is greatly desired.In thisstudy,microspheres-based PHBHO(poly 3-hydroxybutyrate-co-3-hydroxyoctanoate) ofencapsulated bFGF(human basic fibroblast growth factor) were produced using three differentParticle Fabrication technique (electrospinning technique,solvent evaporationtechnique,ultrasonic emulsification technique). Protein probably denaturation and microspheresdiameter was average 10um by electrospinning method. microspheres adhere by solventevaporation method.microspheres-particle size distribution uniform, better dispersion arefabricated by ultrasonic emulsification method.Mean diameter was detected by SEM(scanningelectron microscopy),the nanospheres’diameter were 524.75±67.46nm,The drug loading amountand encapsulation efficiency of bFGF-P(HB-HO)Ns were（1.397±0.018）×10-3% and90.77±1.67%, bFGF-P(HB-HO)NPs can not only sustain release bFGF,but also the vitro releaseprofile displayed that nearly 87.89% in the first 13 days.Then bFGF-P(HB-HO)NPs wereDropped in tissue engineering scaffolds or tissue engineering scaffolds were immersed inbFGF-P(HB-HO)NPs emultions,the scaffolds detected by SEM,the results showed thatnanospheres adhesioned to tissue engineering scaffolds and distributeduniform.microspheres-based PHBHO(poly 3-hydroxybutyrate-co-3-hydroxyoctanoate) ofencapsulated bFGF(human basic fibroblast growth factor) were prepared using ultrasonicemulsification technique, and evaluated its biological effects on cultured rabbit bonemesenchymal stem cells(BMSCs). BMSCs were cultured using whole bone marrow culturemethod. MTT colorimetric assay were used to evaluate the proliferation of the BMSCs.accordingto the different culture medium ingredients,experiment groups were divided into three groups:simple culture medium by adding bFGF (A group), adding bFGF-P (HBHO) NPs (B group), theonly DMEM culture group (C group). effective concentration of the first two groups were set to10,20,50 ng / ml, and five time points of each concentration was tested by MTT method. Theresults were analyzed by statistical software (SPSS13.0). During the first 3 days,the proliferationBMSCs between group A and B had no significance(P>0.05), but much faster than group C(P＜0.01﹚;After 3 to 7 days,between A group and B group had significance(P＜0.01);7 to10days,between B and A,C become much more significant(P＜0.01),but A and C had nosignificance(P>0.05).So bFGF-P (HBHO) NPs release had a more significant biological effectsthan simple bFGF,and could continue to promote their proliferation.