| ObjectiveThe research discusses the relation between Syk and RANKL and alveolar bone remodelingduring orthodontic tooth movement through experimental animal models, TRAP staining andimmunohistochemistry methods of chemical, in order to deeply understand the mechanism oforthodontic tooth movement, and provide a new target of promoting or inhibiting the toothmovement to oral orthodontic work.Methods36 male Wistar rats were randomly divided into 4 groups: the control group (0g group) and theexperiment group 1(30g group), 2(50g group) and 3(100g group), each group is consisted of 9rats. An animal model of orthodontic tooth movement was established, applying. 0g , 30g, 50g,100g mesial force. The rats in the experiment groups were sacrificed after 3 weeks. HE staining,TRAP staining to observe the morphological changes of osteoclasts in rat periodontal tissue. Todetect the expression of Syk and RANKL in alveolar bone tissue in the experimental group andthe control group with Immunohistochemical method. Image analysis system was applied tomeasure the express area and the average gray scale. In addition, SPSS17.0 software was used tocarry out statistical treatment.Results1. To show the pressure in rats side Osteoclast by HE staining and TRAP staining statisticalanalysis: osteoclast number and activity also increased with the rise in the value of orthodonticforce, and maximum appeared under 100g force. But on the aspect of the damage on the pressureside organization made by combination of the number of osteoclast activity and force increase,30g-50g is the best power value.2. RANKL immunohistochemical analysis showed that: the pressure side of the control group,the periodontal tissues of RANKL is mainly distributed in the nucleus of osteoclasts, weakpositive staining. With the increase of the pressure side of the orthodontic force, RANKLexpression is also increased in all experiment groups. Pressure side of the deep-positive staining than the control group, mainly distributed in the pressure side of the periodontal membrane andbone remodeling osteoclasts the nucleus and cytosol. RANKL also expressed in the stromal cellsand periodontal ligament, which showed that RANKL involved in fibroblasts proliferation andactivation of osteoclasts.3. Syk immunohistochemical analysis showed that: in control group, Syk in the pressure side ofalveolar bone osteoclast cell nuclei has positive expression, and the expression rate is low. Syk inthe experiment groups expressed on the nucleus and cytosol of bone remodeling osteoclasts,stromal cells. And it expressed deeper than the control group. With the increase of the pressureside of the orthodontic force, Syk expression also increased, which prompted that Syk involvedin the orthodontic bone remodeling process of bone resorption.4. Through correlation analysis, RANKL and Syk has in great correlation on the distribution ofcells and expressed in different force. We believe that with increasing pressure side of the Syk,RANKL expression is increased.5. 50g force is the best force to produce the best force value of RANKL and Syk.Conclusions1.The experiment infers that 30g-50g of orthodontic force can promote effectively theproliferation and differentiation of osteoclasts, and the smaller damage caused by the periodontaltissue.2. Expression of Syk and RANKL in the pressure side of the periodontal tissues strong.With theincrease of the pressure side of the orthodontic force, Syk and RANKL expression also increased.Which can shows that Syk and RANKL participates in the pressure side of the orthodontic boneremodeling process.3. Through correlation analysis, it showde that Syk in rat alveolar pressure side has a correlationof the expression of RANKL. It inferres that Syk promotes the proliferation and differentiationof osteoclasts through the RANKL pathway.4. 50g is the best force for Syk and RANKL to promote osteoclast proliferation anddifferentiation and bone resorption. |
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