Expression Of ANXA1in Laryngeal Squamous Cell Carcinoma

Objective:Laryngeal squamous cell carcinoma (LSCC) is one of the most common type of tumors,afflicting500000patients worldwide each year.The survival rate for patients with LSCC has remained unchanged in recent years dispite advances in diagnosis and treatment. Experiments have evaluated that, ANXA1expression is closely related with tumor disorders, development, transfer and multi-drug ersistance. Immunocy-tochemlcal detectlon of ANXA1represents a simple, Inexpensive, highly sensltive and specific (100%) assay for diagnosis of hairy cell leukaemia. therefore, this experiment intends to use the immunohistochemistry (IHC) and RT-PCR method to detect the expression of ANXA1in LSCC and noncancerous mucosa and further discusses the expression rules and role in the occurrence and development of LSCC.Methods:Collection of standards-compliant clinical data, IHC and RT-PCR were used to analyze the protein expression of ANXA1from LSCC、 adjacent tissues and premalignant lesions separately. Surgical tissue specimens from43patients with LSCC who consecutively underwent resection of their tumor at the the First Affilliated Hospital of Zhengzhou University were obtained for this prospective study.Noneo of them had received radio or chemotherapy before intervention. Clinically malignant lesions sample and normal adjacent mucosa were also collected. All tissue samples were stored at-80℃less than30minutes until analysis.A portion of the surgical tissue specimen was fixed in buffered10%formaldehyde solutions12h to24h.and embedded in paraffin for use in histological analysis and IHC studies. IHC analysis was performed on a study set of eleven premalignant lesions.Finally, statistical methods used for ststistical analysis of the data.Results:IHC analysis of ANXA1protein expression in patient-matched normal and tumor epithelium from43different patients with primary LSCC. Substantial loss of this39-kd protein in LSCC tissues (33cases,76.7%) and adjacent tissues (7cases,16.3%) was observed. ANXA1protein loss expression in tumor epithelium was higher than patient-matched normal, and its difference was ststistically significant(P <0.05).IHC and RT-PCR analysis of experimental results together showing:ANXA1loss-expresion in LSCC was significantly positively associated with clinicopathologic variables、primary LSCC nantomical site、TNM stage、 locoregional lymph node metastasis and advanced disease stage (P<0.05),and there were no significant correlated with sex in ANXA1loss-expression(P>0.05); ANXA1mRNA loss-expresion in LSCC was significantly positively associated with clinicopathologic variables、primary LSCC nantomical site and TNM stage (P<0.05),and there were no significantly associated with sex、 locoregional lymph node metastasis and advanced disease stage in ANXA1loss-expression(P>0.05); A marked reduction of ANXA1expression was detected as weak staining on the most superficial layers of dysplastic tissue, in marked contrast to the strong ANXA1signal detected in corresponding normal epithelium.Conclusions:1. IHC and RT-PCR detection of the same positive results,which showed that the existence of ANXA1protein loss-expression in LSCC is common phenomenon.2. ANXA1protein loss-expresion in LSCC was significantly associated with clinicopathologic variables、 primary LSCC nantomical site and TNM stage. Hypopharynx tumors with poor differentiation showed a marked reduction of ANXA1protein levels, which suggesting that a marked reduction of ANXA1expression maybe associated with the LSCC malignant behavior.3. There were no significant correlation in ANXA1loss-expression with sex, with locoregional lymph node metastasis and advanced disease stage in ANXA1loss-expression needed further study.4. A marked reduction of ANXA1expression was detected as weak staining on the most superficial layers of dysplastic tissue, which suggesting that ANXA1protein loss-expression maybe associated with the occurrence and development of LSCC.