The Study Of Lidocaine On Reducing Inflammatory Injury During Myocardial Ischemia Reperfusion In Rats

ObjectiveIschemic heart disease has become the most important cause of patient deaths in our country. Recently, with the development and promotion of the difficult complex cardiac and great vessels surgery, for example coronary artery bypass graft, PTCA and heart transplantation, ischemic heart disease has been effective in the treatment and prevention. But the ensuing myocardial ischemia reperfusion injury incidence increased year by year, this seriously affected the clinical effects of these treatments. This study aimed to investigate the protective effect of anti-inflammatory effects of lidocaine on myocardial ischemia reperfusion injury in vivo, explore the impacts by lidocaine poatconditioning on ischemic reperfusion myocardial cardiac function and the degree of neutrophil infiltration, and approach new means for the prevention and treatment of clinical myocardial ischemia reperfusion injury.MethodsEighteen Male and female New Zealand white rabbits weighting2.3-2.5kg were used in this study and randomly divided into three groups (each group of six):sham operation group, ischemic reperfusion group and lidocaine group. The myocardial ischemia/reperfusion injury model of rabbits were reproduced by ligating the left ventricular anterior coronary artery for40minutes and removing the ligation later to reperfuse for120minutes in vivo. Lidocaine was administered through intravenous injection before reperfusion. Left ventricular systolic pressure (LVSP),±p/dtmax of the heart and the level of plasma IL-6and IL-8were detected at40minutes after ischemia and at120minutes after reperfusion. The degree of myocardial injury and neutrophils infiltration were also observed with microscope, and the protein contents of NF-kB were measured by immunohistochemical method.Results1. Lidocain could lighten the degree of myocardial injury. Observed by light microscope myocardial fiber arrangement was more clearly, tightly, moreover bleeding and inflammatory infiltration were lighter obviously.2. Lidocaine could protect the ischemia-reperfusion myocardial function. Compared with those of ischemic reperfusion group, LVSP and±dp/dtmax of the heart were better recovered during reperfusion in lidocaine groups (P<0.05)3. Lidocaine could reduce the release of inflammatory mediators. The IL-6and IL-8concentrations in plasma in lidocaine group were significantly lower than those in the ischemic reperfusion group (P<0.01)4. The protein contents of NF-kB in lidocaine group were significantly lower than those in the ischemic reperfusion group.ConclusionThe results demonstrate that lidocaine could effectively reduce myocardial structural damage, protect cardiac function, inhibit inflammatory response and attenuate reperfusion injury in ischemic heart.